Home LiteratureArticle Details
PMID: 2703463 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Identification of a gene linked to Rhizobium meliloti ntrA whose product is homologous to a family to ATP-binding proteins.

Journal of bacteriology ·Vol. 171 ·No. 4 ·1989-04-00 ·Pages 1932-41

Albright LM, Ronson CW, Nixon BT, Ausubel FM

Abstract

The ntrA gene of Rhizobium meliloti has recently been identified and shown to be required for a diverse set of metabolic functions (C. W. Ronson, B. T. Nixon, L. M. Albright, and F. M. Ausubel, J. Bacteriol. 169:2424-2431, 1987). As a result of sequencing the ntrA gene and its flanking regions from R. meliloti, we identified an open reading frame directly upstream of ntrA, ORF1, whose predicted product is homologous to a superfamily of ATP-binding proteins involved in transport, cell division, nodulation, and DNA repair. The homology of ORF1 to this superfamily and its proximity to ntrA led us to investigate its role in symbiosis by mutagenesis and expression studies. We were unable to isolate an insertion mutation in ORF1, suggesting that ORF1 may code for an essential function. We identified the start of transcription for the ntrA gene in vegetative cells and bacteroids and showed that ORF1 and ntrA are transcriptionally unlinked. ORF1 appears to be in an operon with one or more upstream genes.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Carrier Proteins/genetics Chromosome Mapping Codon DNA Mutational Analysis Molecular Sequence Data Multigene Family Peptide Chain Initiation, Translational Rhizobium/genetics Transcription, Genetic
Chemicals
Bacterial Proteins Carrier Proteins Codon
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Albright L M
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Ronson C W
Nixon B T
Ausubel F M
References (41)
41 references, click to expand
  1. Analysis of the 5' regulatory region of the gene for delta-aminolevulinic acid synthetase of Rhizobium meliloti.
    Nucleic Acids Res. 1985 Aug 26;13(16):5965-76 PMID: 2994020
  2. Developmental regulation of nodule-specific genes in alfalfa root nodules.
    Mol Plant Microbe Interact. 1988 Feb;1(2):66-74 PMID: 2908768
  3. The nucleotide sequence of the nitrogen-regulation gene ntrA of Klebsiella pneumoniae and comparison with conserved features in bacterial RNA polymerase sigma factors.
    Nucleic Acids Res. 1985 Nov 11;13(21):7607-20 PMID: 2999700
  4. Products of nitrogen regulatory genes ntrA and ntrC of enteric bacteria activate glnA transcription in vitro: evidence that the ntrA product is a sigma factor.
    Proc Natl Acad Sci U S A. 1985 Nov;82(22):7525-9 PMID: 2999766
  5. Transcription of glnA by purified Escherichia coli components: core RNA polymerase and the products of glnF, glnG, and glnL.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8453-7 PMID: 2867543
  6. Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP.
    Nucleic Acids Res. 1986 Feb 11;14(3):1319-24 PMID: 3951988
  7. Bacterial periplasmic transport systems: structure, mechanism, and evolution.
    Annu Rev Biochem. 1986;55:397-425 PMID: 3527048
  8. Secretion of haemolysin by Escherichia coli.
    Curr Top Microbiol Immunol. 1986;125:159-81 PMID: 3017638
  9. The nodI gene product of Rhizobium leguminosarum is closely related to ATP-binding bacterial transport proteins; nucleotide sequence analysis of the nodI and nodJ genes.
    Gene. 1986;43(1-2):95-101 PMID: 3019841
  10. A family of related ATP-binding subunits coupled to many distinct biological processes in bacteria.
    Nature. 1986 Oct 2-8;323(6087):448-50 PMID: 3762694
  11. Domainal evolution of a prokaryotic DNA repair protein and its relationship to active-transport proteins.
    Nature. 1986 Oct 2-8;323(6087):451-3 PMID: 3762695
  12. Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli.
    Proc Natl Acad Sci U S A. 1973 May;70(5):1514-8 PMID: 4268097
  13. Different mechanisms of energy coupling for the shock-sensitive and shock-resistant amino acid permeases of Escherichia coli.
    J Biol Chem. 1974 Dec 25;249(24):7747-55 PMID: 4279250
  14. Transposition of R factor genes to bacteriophage lambda.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3628-32 PMID: 1059152
  15. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  16. Genetic analysis of bacteriophage P4 using P4-plasmid ColE1 hybrids.
    Mol Gen Genet. 1980 Feb;177(3):399-412 PMID: 6929401
  17. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
    J Mol Biol. 1980 Apr;138(2):179-207 PMID: 6997493
  18. A general method for site-directed mutagenesis in prokaryotes.
    Nature. 1981 Jan 1;289(5793):85-8 PMID: 6256652
  19. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals.
    J Bacteriol. 1980 Aug;143(2):971-80 PMID: 6162838
  20. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51 PMID: 7012838
  21. Oligonucleotide directed mutagenesis of the human beta-globin gene: a general method for producing specific point mutations in cloned DNA.
    Nucleic Acids Res. 1981 Aug 11;9(15):3647-56 PMID: 7279669
  22. Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis.
    J Bacteriol. 1982 Jan;149(1):114-22 PMID: 6274841
  23. Characterization of translational initiation sites in E. coli.
    Nucleic Acids Res. 1982 May 11;10(9):2971-96 PMID: 7048258
  24. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.
    Nucleic Acids Res. 1982 Aug 11;10(15):4731-51 PMID: 7133997
  25. Establishing homologies in protein sequences.
    Methods Enzymol. 1983;91:524-45 PMID: 6855599
  26. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  27. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  28. Graphic methods to determine the function of nucleic acid sequences.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):521-38 PMID: 6364040
  29. Bacteriophage T4 late promoters: mapping 5' ends of T4 gene 23 mRNAs.
    EMBO J. 1982;1(1):107-14 PMID: 6201352
  30. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
    EMBO J. 1982;1(8):945-51 PMID: 6329717
  31. ATP-binding sites in the membrane components of histidine permease, a periplasmic transport system.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7333-7 PMID: 6239289
  32. Nucleotide binding by membrane components of bacterial periplasmic binding protein-dependent transport systems.
    EMBO J. 1985 Apr;4(4):1033-9 PMID: 3926486
  33. A new cell division operon in Escherichia coli.
    Mol Gen Genet. 1986 Oct;205(1):134-45 PMID: 3025556
  34. Processing of the T-DNA of Agrobacterium tumefaciens generates border nicks and linear, single-stranded T-DNA.
    J Bacteriol. 1987 Mar;169(3):1046-55 PMID: 3029014
  35. Identification and characterization of the Rhizobium meliloti ntrC gene: R. meliloti has separate regulatory pathways for activation of nitrogen fixation genes in free-living and symbiotic cells.
    J Bacteriol. 1987 Apr;169(4):1423-32 PMID: 2881918
  36. Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions.
    J Bacteriol. 1987 Jun;169(6):2424-31 PMID: 3034856
  37. Molecular characterization of the oligopeptide permease of Salmonella typhimurium.
    J Mol Biol. 1987 May 5;195(1):125-42 PMID: 2821267
  38. The Escherichia coli cell division proteins FtsY, FtsE and FtsX are inner membrane-associated.
    Mol Gen Genet. 1987 Dec;210(3):504-8 PMID: 3323846
  39. An altered pattern of cross-resistance in multidrug-resistant human cells results from spontaneous mutations in the mdr1 (P-glycoprotein) gene.
    Cell. 1988 May 20;53(4):519-29 PMID: 2897240
  40. The ndvA gene product of Rhizobium meliloti is required for beta-(1----2)glucan production and has homology to the ATP-binding export protein HlyB.
    J Bacteriol. 1988 Aug;170(8):3523-30 PMID: 3042754
  41. Regions of broad-host-range plasmid RK2 involved in replication and stable maintenance in nine species of gram-negative bacteria.
    J Bacteriol. 1985 Oct;164(1):446-55 PMID: 4044529
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.4.1932-1941.1989
Published
1989-04-00
Pages
1932-41
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209842
Subset
IM
Databases
GENBANK
M24926
External Links
PubMed source DOI: 10.1128/jb.171.4.1932-1941.1989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2