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PMID: 270713 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

2-Aminopurine-induced mutagenesis in T4 bacteriophage: a model relating mutation frequency to 2-aminopurine incorporation in DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 11 ·1977-11-00 ·Pages 4806-10

Goodman MF, Hopkins R, Gore WC

Abstract

We measured the in vivo incorporation of 2-aminopurine into DNA of T4 bacteriophage allelic for gene 43 (DNA polymerase), mutator (L56), 43+, and antimutator (L141). The magnitude of incorporation (mol/mol of Thy) was 1/1500 in L56, 1/1600 in 43+, and 1/8900 in L141. The incorporation ratio L56:43+:L141 in vivo was equal to that mediated by the purified DNA polymerases of these allelic phages in vitro. A model for 2-aminopurine-induced A-T in equilibrium G-C transitions is discussed. The model is used to predict the magnitudes of replication errors (C mispairing with a template 2-aminopurine) and incorporation errors (2-aminopurine mispairing with a template C) per round of replication and to investigate the asymmetry in 2-aminopurine-induced transitions favoring the A-T leads to G-C pathway over G-C leads to A-T. We suggest that the fidelity of L56 and L141 DNA polymerases exemplifies one-step and two-step editing, respectively.

MeSH Terms
2-Aminopurine/pharmacology Adenine/analogs & derivatives Aminopyrine/metabolism Coliphages/drug effects,genetics DNA, Viral/biosynthesis Models, Biological Mutagens
Chemicals
DNA, Viral Mutagens Aminopyrine 2-Aminopurine Adenine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Goodman M F
Hopkins R
Gore W C
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.11.4806
Published
1977-11-00
Pages
4806-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432044
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.11.4806 PMC Full Text View journal details
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