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PMID: 2787512 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection of mRNA for the tax1/rex1 gene of human T-cell leukemia virus type I in fresh peripheral blood mononuclear cells of adult T-cell leukemia patients and viral carriers by using the polymerase chain reaction.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 14 ·1989-07-00 ·Pages 5620-4

Kinoshita T, Shimoyama M, Tobinai K, Ito M, Ito S, Ikeda S, Tajima K, Shimotohno K, Sugimura T

Abstract

Expression of human T-cell leukemia virus type I (HTLV-I) is not detectable by immunofluorescence analysis or RNA blot analysis in most fresh peripheral blood mononuclear cells of patients with adult T-cell leukemia or of asymptomatic HTLV-I carriers. However, in this work, mRNA for the HTLV-I tax1/rex1 genes was detected in fresh peripheral blood mononuclear cells of adult T-cell leukemia patients and asymptomatic HTLV-I carriers by using reverse transcription followed by the polymerase chain reaction. By using fresh peripheral blood mononuclear cells, the expression of tax1/rex1 mRNA was detected in five of the six adult T-cell leukemia patients and four of the eight HTLV-I carriers examined. The amounts of tax1/rex1 mRNA detected corresponded to approximately 10(5) to 10(6) times less than that in the HTLV-I-infected MT-2 cell line. These results indicate that, in some individuals infected with HTLV-I, the provirus in circulating blood cells is transcribed in vivo. Thus the expression of viral antigens in circulating blood cells in vivo is suggested.

MeSH Terms
Adult Aged Base Sequence Carrier State Cell Line DNA-Directed DNA Polymerase Female Gene Amplification Genes, Viral HTLV-I Antibodies/analysis HTLV-I Antigens/genetics HTLV-I Infections/blood,microbiology Human T-lymphotropic virus 1/genetics Humans Male Middle Aged Molecular Sequence Data Monocytes/microbiology RNA, Messenger/blood,genetics RNA, Viral/blood,genetics Trans-Activators Transcription Factors/genetics Transcription, Genetic
Chemicals
HTLV-I Antibodies HTLV-I Antigens RNA, Messenger RNA, Viral Trans-Activators Transcription Factors DNA-Directed DNA Polymerase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Kinoshita T
Virology Division, National Cancer Center Research Institute, Tokyo, Japan.
Shimoyama M
Tobinai K
Ito M
Ito S
Ikeda S
Tajima K
Shimotohno K
Sugimura T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.14.5620
Published
1989-07-00
Pages
5620-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297674
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.14.5620 PMC Full Text View journal details
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