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PMID: 2796992 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The efficiency of 3'-end formation contributes to the relative levels of different histone mRNAs.

Molecular and cellular biology ·Vol. 9 ·No. 8 ·1989-08-00 ·Pages 3499-508

Liu TJ, Levine BJ, Skoultchi AI, Marzluff WF

Abstract

Sequences at both the 5' and 3' ends of mouse histone genes contribute to the expression of individual genes. The 3' sequences required for high expression of the mouse H2a-614 gene are the same as the sequences required for 3'-end formation. When these sequences were substituted for the 3' end of the poorly expressed H2a-291 gene, expression of the H2a-291 gene was increased fivefold. A 65-nucleotide fragment containing the H2a-614 3' processing signal increased expression of the H2a-291 gene when it was placed in the proper orientation downstream of the H2a-291 3' end. The only mRNAs that accumulated from this gene ended at the H2a-291 3' end, which suggests that the transcript is sequentially processed. In an in vitro processing system, the different histone 3' ends showed different processing efficiencies, which correlated with their expression in cells. These results suggest that the efficiency of processing is important in determining the steady-state levels of individual mouse histone mRNAs.

MeSH Terms
Animals Base Sequence Cell Line Cells, Cultured Cloning, Molecular DNA Mutational Analysis Gene Expression Regulation Histones/genetics Mice Molecular Sequence Data Nucleic Acid Conformation Plasmids Poly A/metabolism RNA Processing, Post-Transcriptional RNA, Messenger/analysis Sequence Homology, Nucleic Acid Single-Strand Specific DNA and RNA Endonucleases
Chemicals
Histones RNA, Messenger Poly A Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Liu T J
Department of Chemistry, Florida State University, Tallahassee 32306.
Levine B J
Skoultchi A I
Marzluff W F
References (31)
31 references, click to expand
  1. Intragenic pausing and anti-sense transcription within the murine c-myc locus.
    EMBO J. 1986 Nov;5(11):2859-65 PMID: 3024965
  2. snRNP mediators of 3' end processing: functional fossils?
    Trends Biochem Sci. 1988 Nov;13(11):447-51 PMID: 2908086
  3. Translation is required for regulation of histone mRNA degradation.
    Cell. 1987 Feb 27;48(4):615-26 PMID: 3028643
  4. The organization and expression of histone gene families.
    Cell. 1981 Aug;25(2):301-13 PMID: 6793234
  5. Structure and expression in L-cells of a cloned H4 histone gene of the mouse.
    J Mol Biol. 1981 Oct 5;151(4):607-25 PMID: 6276563
  6. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  7. Histone mRNA concentrations are regulated at the level of transcription and mRNA degradation.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):1849-53 PMID: 6572946
  8. Regulation of human histone gene expression: kinetics of accumulation and changes in the rate of synthesis and in the half-lives of individual histone mRNAs during the HeLa cell cycle.
    Mol Cell Biol. 1983 Apr;3(4):539-50 PMID: 6406835
  9. Structure of a cluster of mouse histone genes.
    Nucleic Acids Res. 1983 Oct 11;11(19):6679-97 PMID: 6314253
  10. Regulation of histone mRNA production and stability in serum-stimulated mouse 3T6 fibroblasts.
    Mol Cell Biol. 1983 Nov;3(11):1920-9 PMID: 6656760
  11. Rapid reversible changes in the rate of histone gene transcription and histone mRNA levels in mouse myeloma cells.
    Mol Cell Biol. 1984 Feb;4(2):351-7 PMID: 6700595
  12. Transcription termination and 3' processing: the end is in site!
    Cell. 1985 Jun;41(2):349-59 PMID: 2580642
  13. Differential expression of two clusters of mouse histone genes.
    J Mol Biol. 1985 May 25;183(2):179-94 PMID: 2989540
  14. A block to elongation is largely responsible for decreased transcription of c-myc in differentiated HL60 cells.
    Nature. 1986 Jun 12-18;321(6071):702-6 PMID: 3520340
  15. High-frequency transfection of CHO cells using polybrene.
    Somat Cell Mol Genet. 1986 May;12(3):237-44 PMID: 3459255
  16. Generation of histone mRNA 3' ends by endonucleolytic cleavage of the pre-mRNA in a snRNP-dependent in vitro reaction.
    EMBO J. 1986 Jun;5(6):1319-26 PMID: 3015597
  17. Regulated expression of a chimeric histone gene introduced into mouse fibroblasts.
    Mol Cell Biol. 1985 Sep;5(9):2316-24 PMID: 2874481
  18. Globin gene transcripts can utilize histone gene 3' end processing signals.
    Nucleic Acids Res. 1986 Sep 11;14(17):7059-70 PMID: 3463933
  19. Transcriptional arrest within the first exon is a fast control mechanism in c-myc gene expression.
    Nucleic Acids Res. 1986 Nov 11;14(21):8331-46 PMID: 3537956
  20. Expression of mouse histone genes: transcription into 3' intergenic DNA and cryptic processing sites downstream from the 3' end of the H3 gene.
    Mol Cell Biol. 1987 Mar;7(3):1039-47 PMID: 2882414
  21. Mouse histone H2A and H2B genes: four functional genes and a pseudogene undergoing gene conversion with a closely linked functional gene.
    Nucleic Acids Res. 1987 Apr 10;15(7):3023-39 PMID: 3562244
  22. Both conserved signals on mammalian histone pre-mRNAs associate with small nuclear ribonucleoproteins during 3' end formation in vitro.
    Mol Cell Biol. 1987 May;7(5):1663-72 PMID: 2955216
  23. RNA 3' processing regulates histone mRNA levels in a mammalian cell cycle mutant. A processing factor becomes limiting in G1-arrested cells.
    EMBO J. 1987 Jun;6(6):1721-6 PMID: 3608992
  24. Coupling of replication type histone mRNA levels to DNA synthesis requires the stem-loop sequence at the 3' end of the mRNA.
    Proc Natl Acad Sci U S A. 1987 Sep;84(17):6189-93 PMID: 2888112
  25. Differential expression of c-myb mRNA in murine B lymphomas by a block to transcription elongation.
    Science. 1987 Sep 18;237(4821):1473-6 PMID: 3498214
  26. Identification of the human U7 snRNP as one of several factors involved in the 3' end maturation of histone premessenger RNA's.
    Science. 1987 Dec 18;238(4834):1682-7 PMID: 2825355
  27. Differential expression of individual members of the histone multigene family due to sequences in the 5' and 3' regions of the genes.
    Mol Cell Biol. 1988 May;8(5):1887-95 PMID: 3386629
  28. Specific contacts between mammalian U7 snRNA and histone precursor RNA are indispensable for the in vitro 3' RNA processing reaction.
    EMBO J. 1988 Mar;7(3):801-8 PMID: 3396543
  29. 3' processing of pre-mRNA plays a major role in proliferation-dependent regulation of histone gene expression.
    Nucleic Acids Res. 1988 Oct 25;16(20):9399-414 PMID: 3141900
  30. Multiple regulatory steps control histone mRNA concentrations.
    Trends Biochem Sci. 1988 Feb;13(2):49-52 PMID: 3070846
  31. Sequences of four mouse histone H3 genes: implications for evolution of mouse histone genes.
    J Mol Evol. 1986;23(3):242-9 PMID: 3027355
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.8.3499-3508.1989
Published
1989-08-00
Pages
3499-508
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362397
Subset
IM
Grants
NCI NIH HHS · CA13330 · United States
NCI NIH HHS · CA16368 · United States
NIGMS NIH HHS · GM29832 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.8.3499-3508.1989 PMC Full Text View journal details
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