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PMID: 2798416 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Conservation of an immunoglobulin variable-region gene family indicates a specific, noncoding function.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 19 ·1989-10-00 ·Pages 7460-4

Tutter A, Riblet R

Abstract

Blot-hybridization and DNA sequence analyses reveal the particular evolutionary conservation of a group of immunoglobulin heavy-chain variable-region (VH) genes in all mammalian species examined. These particular genes are group III genes--the VH7183 family in the mouse and the homologous VH III family in human. This conservation is localized to sequences encoding framework regions 1 and 3 of the antibody variable region and is exerted at the nucleotide level. Because selection acting at the amino acid level alone cannot explain the conservation of these sequences, these sequences must have a noncoding function. The preferential rearrangement of VH7183 and VH III genes, together with the similarity of the conserved sequences to elements implicated in recombination in other systems, suggest that these sequences function to target the series of rearrangements that assemble complete immunoglobulin genes.

MeSH Terms
Animals Base Sequence Biological Evolution DNA/genetics Gene Rearrangement Genes, Immunoglobulin Humans Immunoglobulin Variable Region/genetics Mice Molecular Sequence Data Multigene Family Nucleic Acid Hybridization Phylogeny Software Species Specificity
Chemicals
Immunoglobulin Variable Region DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tutter A
Medical Biology Institute, La Jolla, CA 92037.
Riblet R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.19.7460
Published
1989-10-00
Pages
7460-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298084
Subset
IM
Grants
NIAID NIH HHS · AI23548 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.19.7460 PMC Full Text View journal details
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