Home LiteratureArticle Details
PMID: 2820716 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The origin of bidirectional DNA replication in polyoma virus.

The EMBO journal ·Vol. 6 ·No. 7 ·1987-07-00 ·Pages 2011-8

Hendrickson EA, Fritze CE, Folk WR, DePamphilis ML

Abstract

The nucleotide locations of RNA-p-DNA covalent linkages in polyoma virus (PyV) replicating DNA were mapped in the region containing the genetically required origin of DNA replication (ori). These linkages mark the initiation sites for RNA-primed DNA synthesis. A clear transition was identified between the presence of these linkages (discontinuous DNA synthesis) and their absence (continuous DNA synthesis) on each strand of ori. This demonstrated that PyV DNA replication, like simian virus 40 (SV40), is semi-discontinuous, and thus revealed the location of the origin of bidirectional DNA replication (OBR). The transition site on the template encoding PyV late mRNA occurred at the junction of ori-core and T-antigen binding site A. This was essentially the same site as previously observed in SV40 (Hay and DePamphilis, 1982). However, in contrast to SV40, the transition site on the template encoding PyV early mRNA was displaced towards the late gene side of ori. This resulted in a 16 nucleotide gap within ori in which no RNA-p-DNA linkages were observed on either strand. A model for the initiation of PyV DNA replication is presented.

MeSH Terms
Base Sequence Coliphages/genetics DNA Replication Genes, Viral Genetic Vectors Kinetics Molecular Sequence Data Polyomavirus/genetics Simian virus 40/genetics
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hendrickson E A
Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115.
Fritze C E
Folk W R
DePamphilis M L
References (48)
48 references, click to expand
  1. Sequence specificity for the initiation of RNA-primed simian virus 40 DNA synthesis in vivo.
    J Mol Biol. 1984 May 15;175(2):131-57 PMID: 6202875
  2. DNA binding activity of polyoma virus large tumor antigen.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1941-5 PMID: 6326093
  3. Simian virus 40 DNA replication in vitro.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6973-7 PMID: 6095264
  4. Multiple initiation sites of DNA replication flanking the origin region of lambda dv genome.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7402-6 PMID: 6095292
  5. Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma.
    Cell. 1984 Dec;39(3 Pt 2):653-62 PMID: 6096017
  6. Location of sequences in polyomavirus DNA that are required for early gene expression in vivo and in vitro.
    Mol Cell Biol. 1984 Dec;4(12):2594-609 PMID: 6098813
  7. Polyomavirus enhancer contains multiple redundant sequence elements that activate both DNA replication and gene expression.
    Mol Cell Biol. 1985 Apr;5(4):649-58 PMID: 2985964
  8. DNA primase-DNA polymerase alpha from simian cells: sequence specificity of initiation sites on simian virus 40 DNA.
    Mol Cell Biol. 1985 May;5(5):1170-83 PMID: 2582240
  9. The distribution and properties of RNA primed initiation sites of DNA synthesis at the replication origin of Escherichia coli chromosome.
    Nucleic Acids Res. 1985 Oct 11;13(19):6847-66 PMID: 2414732
  10. Functional interactions of the simian virus 40 core origin of replication with flanking regulatory sequences.
    J Virol. 1986 Jan;57(1):138-44 PMID: 3001340
  11. Guanine nucleotide contacts within viral DNA sequences bound by polyomavirus large T antigen.
    J Virol. 1986 Feb;57(2):505-14 PMID: 3003383
  12. DNA binding site for a factor(s) required to initiate simian virus 40 DNA replication.
    Proc Natl Acad Sci U S A. 1986 Mar;83(6):1646-50 PMID: 3006062
  13. Nuclear activity from F9 embryonal carcinoma cells binding specifically to the enhancers of wild-type polyoma virus and PyEC mutant DNAs.
    Nucleic Acids Res. 1986 Apr 11;14(7):2845-61 PMID: 2421253
  14. Role of DNA polymerase alpha and DNA primase in simian virus 40 DNA replication in vitro.
    Proc Natl Acad Sci U S A. 1986 May;83(9):2869-73 PMID: 3010320
  15. Species-specific in vitro synthesis of DNA containing the polyoma virus origin of replication.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6347-51 PMID: 3018726
  16. DNA helicase activity of SV40 large tumor antigen.
    EMBO J. 1986 Aug;5(8):1939-44 PMID: 3019672
  17. Interaction of a nuclear factor with the polyomavirus enhancer region.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8550-4 PMID: 3022296
  18. Functional organization of the simian virus 40 origin of DNA replication.
    Mol Cell Biol. 1986 Apr;6(4):1117-28 PMID: 3023870
  19. Interaction of distinct nuclear proteins with sequences controlling the expression of polyomavirus early genes.
    Mol Cell Biol. 1986 May;6(5):1401-11 PMID: 3023889
  20. Domain structure of the simian virus 40 core origin of replication.
    Mol Cell Biol. 1986 May;6(5):1663-70 PMID: 3023900
  21. Simian virus 40 DNA replication: functional organization of regulatory elements.
    Mol Cell Biol. 1986 Sep;6(9):3086-93 PMID: 3023962
  22. Bidirectional promoter elements of simian virus 40 are required for efficient replication of the viral DNA.
    Mol Cell Biol. 1986 Oct;6(10):3513-22 PMID: 3025597
  23. Initiation of simian virus 40 DNA replication in vitro: aphidicolin causes accumulation of early-replicating intermediates and allows determination of the initial direction of DNA synthesis.
    Mol Cell Biol. 1986 Nov;6(11):3815-25 PMID: 3025613
  24. T-antigen-DNA polymerase alpha complex implicated in simian virus 40 DNA replication.
    Mol Cell Biol. 1986 Nov;6(11):4077-87 PMID: 3025630
  25. Simian virus 40 (SV40) DNA replication: SV40 large T antigen unwinds DNA containing the SV40 origin of replication.
    Proc Natl Acad Sci U S A. 1987 Jan;84(1):16-20 PMID: 3025851
  26. Molecular analysis of the interaction between an enhancer binding factor and its DNA target.
    Nucleic Acids Res. 1986 Dec 22;14(24):9595-611 PMID: 3027657
  27. Topography of simian virus 40 A protein-DNA complexes: arrangement of protein bound to the origin of replication.
    J Virol. 1983 Apr;46(1):151-61 PMID: 6298452
  28. Initiator RNA in discontinuous polyoma DNA synthesis.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4901-5 PMID: 4373733
  29. Assay of RNA-linked nascent DNA pieces with polynucleotide kinase.
    Biochem Biophys Res Commun. 1975 Feb 17;62(4):1018-24 PMID: 164190
  30. RNA primers in SV40 DNA replication: identification of transient RNA-DNA covalent linkages in replicating DNA.
    Biochemistry. 1977 Nov 15;16(23):4990-8 PMID: 199246
  31. RNA primers in Simian virus 40 DNA replication. II. Distribution of 5' terminal oligoribonucleotides in nascent DNA.
    J Mol Biol. 1977 Nov 5;116(3):549-67 PMID: 201764
  32. Asymmetric Okazaki piece synthesis during replication of simian virus 40 DNA in vivo.
    Cell. 1977 Dec;12(4):1029-43 PMID: 202391
  33. The binding site on SV40 DNA for a T antigen-related protein.
    Cell. 1978 Jan;13(1):165-79 PMID: 202398
  34. Okazaki pieces grow opposite to the replication fork direction during simian virus 40 DNA replication.
    Nucleic Acids Res. 1978 Jul;5(7):2535-45 PMID: 209411
  35. Replication of polyoma DNA in isolated nuclei. VII. Initiator RNA synthesis during nucleotide depletion.
    J Mol Biol. 1979 Apr 15;129(3):393-409 PMID: 222912
  36. Mutational analysis of the simian virus 40 replicon: pseudorevertants of mutants with a defective replication origin.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6128-31 PMID: 230499
  37. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  38. The nucleotide sequence and restriction enzyme sites of the polyoma genome.
    Nucleic Acids Res. 1980 Feb 25;8(4):855-60 PMID: 6253927
  39. Territorial limits and functional anatomy of the simian virus 40 replication origin.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):381-5 PMID: 6281769
  40. Replication of animal mitochondrial DNA.
    Cell. 1982 Apr;28(4):693-705 PMID: 6178513
  41. Initiation of SV40 DNA replication in vivo: location and structure of 5' ends of DNA synthesized in the ori region.
    Cell. 1982 Apr;28(4):767-79 PMID: 6178514
  42. Regulatory mutants of simian virus 40. Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes.
    J Mol Biol. 1982 Apr 15;156(3):531-48 PMID: 6288959
  43. Comparison of the DNA sequence of the Crawford small-plaque variant of polyomavirus with those of polyomaviruses A2 and strain 3.
    J Virol. 1983 Nov;48(2):472-80 PMID: 6312103
  44. Deletion mapping of DNA regions required for SV40 early region promoter function in vivo.
    J Mol Appl Genet. 1982;1(5):457-81 PMID: 6296253
  45. Asynchronous bidirectional replication of polyoma virus DNA.
    J Virol. 1982 Sep;43(3):885-95 PMID: 6292468
  46. Initiation of simian virus 40 DNA replication in vitro.
    J Virol. 1983 Nov;48(2):481-91 PMID: 6312104
  47. Simian virus 40 mutant T antigens with relaxed specificity for the nucleotide sequence at the viral DNA origin of replication.
    J Virol. 1984 Feb;49(2):386-93 PMID: 6319739
  48. Essential nucleotides in the polyomavirus origin region.
    J Virol. 1984 Aug;51(2):437-44 PMID: 6086955
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-07-00
Pages
2011-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553590
Subset
IM
Grants
PHS HHS · 1R016M37136-01 · United States
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2