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PMID: 2821887 Published · ppublish English Journal Article

Homology of a transferable tetracycline resistance determinant of Clostridium difficile with Streptococcus (Enterococcus) faecalis transposon Tn916.

Antimicrobial agents and chemotherapy ·Vol. 31 ·No. 7 ·1987-07-00 ·Pages 1033-8

Hächler H, Kayser FH, Berger-Bächi B

Abstract

In several tetracycline-resistant (Tetr) Clostridium difficile strains, homology with the Tn916 part of plasmid pAM120 DNA was observed. This 15-kilobase transposon, carrying a Tetr determinant, was originally found in Streptococcus (Enterococcus) faecalis. Hybridization experiments revealed that at least six of seven HincII fragments of Tn916, representing greater than 95% of its length, showed homology with DNA of Tetr C. difficile strains. Therefore, a close relationship of the C. difficile Tetr-determining element with the entire Tn916 transposon can be assumed, although differences were observed concerning the number of HindIII cleavage sites within the transposon. In addition to strong hybridization of Tetr determinants of C. difficile with Tn916, weak signals were detected when DNA of Tets C. difficile was hybridized with Tn916. These weak signals could be attributed to a single internal HincII fragment of Tn916.

MeSH Terms
Clostridium/drug effects,genetics DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial/analysis Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Deoxyribonucleases, Type II Site-Specific Drug Resistance, Microbial/genetics Electrophoresis, Agar Gel Enterococcus faecalis/genetics Nucleic Acid Hybridization R Factors Sequence Homology, Nucleic Acid Tetracycline/pharmacology
Chemicals
DNA Transposable Elements DNA, Bacterial DNA Restriction Enzymes Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Deoxyribonucleases, Type II Site-Specific GTYRAC-specific type II deoxyribonucleases Tetracycline
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hächler H
Department of Medical Microbiology, University of Zurich, Switzerland.
Kayser F H
Berger-Bächi B
References (30)
30 references, click to expand
  1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  2. Genetic characterization of a Clostridium difficile erythromycin-clindamycin resistance determinant that is transferable to Staphylococcus aureus.
    Antimicrob Agents Chemother. 1987 Jul;31(7):1039-45 PMID: 2821888
  3. Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia.
    N Engl J Med. 1978 Mar 9;298(10):531-4 PMID: 625309
  4. Identification of Clostridium difficile as a cause of pseudomembranous colitis.
    Br Med J. 1978 Mar 18;1(6114):695 PMID: 630301
  5. Clostridium difficile and the aetiology of pseudomembranous colitis.
    Lancet. 1978 May 20;1(8073):1063-6 PMID: 77366
  6. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  7. In vitro susceptibility of Clostridium difficile isolates from patients with antibiotic-associated diarrhea or colitis.
    Antimicrob Agents Chemother. 1980 Apr;17(4):695-8 PMID: 7396460
  8. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of "conjugal" transfer in the absence of a conjugative plasmid.
    J Bacteriol. 1981 Jan;145(1):494-502 PMID: 6257641
  9. Transferable tetracycline resistance in Clostridium difficile.
    Antimicrob Agents Chemother. 1981 Jun;19(6):997-1003 PMID: 7271279
  10. Heterogeneity of tetracycline resistance determinants in Streptococcus.
    J Bacteriol. 1982 Mar;149(3):995-1004 PMID: 6801018
  11. Macrolide, lincosamide, streptogramin and tetracycline transferable resistance in the Bacteroides fragilis group.
    J Antimicrob Chemother. 1981 Dec;8 Suppl D:87-94 PMID: 6802794
  12. Construction of overproducers of the bacteriophage 434 repressor and cro proteins.
    J Mol Appl Genet. 1981;1(2):139-47 PMID: 6286819
  13. Mapping of Streptococcus faecalis plasmids pAD1 and pAD2 and studies relating to transposition of Tn917.
    J Bacteriol. 1982 Dec;152(3):1220-30 PMID: 6292164
  14. Investigation of an outbreak of antibiotic-associated colitis by various typing methods.
    J Clin Microbiol. 1982 Dec;16(6):1096-101 PMID: 7161375
  15. Transferable resistance to clindamycin, erythromycin, and tetracycline in Clostridium difficile.
    Antimicrob Agents Chemother. 1983 May;23(5):784-6 PMID: 6870225
  16. Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid from Staphylococcus aureus.
    Plasmid. 1983 Nov;10(3):251-9 PMID: 6657777
  17. Frequency of tetracycline resistance determinant classes among lactose-fermenting coliforms.
    Antimicrob Agents Chemother. 1983 Dec;24(6):835-40 PMID: 6660856
  18. Plasmid-mediated tetracycline resistance in Haemophilus ducreyi.
    Antimicrob Agents Chemother. 1984 Feb;25(2):187-90 PMID: 6324665
  19. Biochemical and genetic characterization of the tet determinant of Bacillus plasmid pAB124.
    J Bacteriol. 1984 Apr;158(1):134-40 PMID: 6325384
  20. Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning of genes from gram-positive bacteria.
    J Bacteriol. 1984 Jul;159(1):214-21 PMID: 6330031
  21. Homogeneity of transferable tetracycline-resistance determinants in Haemophilus species.
    J Infect Dis. 1984 Jun;149(6):1028-9 PMID: 6330236
  22. A conjugative transposon (Tn919) in Streptococcus sanguis.
    Infect Immun. 1985 Feb;47(2):415-20 PMID: 2981772
  23. Physical characterization of the Clostridium perfringens tetracycline-chloramphenicol resistance plasmid pIP401.
    Ann Microbiol (Paris). 1984 Nov-Dec;135B(3):269-82 PMID: 6099703
  24. Streptococcus faecalis sex pheromone (cAM373) also produced by Staphylococcus aureus and identification of a conjugative transposon (Tn918).
    J Bacteriol. 1985 Jun;162(3):1212-20 PMID: 2987186
  25. Tetracycline-resistant Mycoplasma hominis strains contain streptococcal tetM sequences.
    Antimicrob Agents Chemother. 1985 Jul;28(1):141-3 PMID: 2994555
  26. Dissemination of the tetM tetracycline resistance determinant to Ureaplasma urealyticum.
    Antimicrob Agents Chemother. 1986 Feb;29(2):350-2 PMID: 3521481
  27. Studies of antimicrobial resistance genes using DNA probes.
    Antimicrob Agents Chemother. 1986 May;29(5):721-5 PMID: 3524426
  28. High-level tetracycline resistance in Neisseria gonorrhoeae is result of acquisition of streptococcal tetM determinant.
    Antimicrob Agents Chemother. 1986 Nov;30(5):664-70 PMID: 3099640
  29. Heterogeneity of tetracycline resistance determinants.
    Plasmid. 1980 Mar;3(2):99-108 PMID: 6927764
  30. Identification and characterization of a small sequence located at two sites on the amplifiable tetracycline resistance plasmid pAMalpha1 in Streptococcus faecalis.
    J Bacteriol. 1977 Jan;129(1):400-6 PMID: 401496
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.31.7.1033
Published
1987-07-00
Pages
1033-8
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC174867
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.31.7.1033 PMC Full Text View journal details
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