Home LiteratureArticle Details
PMID: 2821901 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vivo formation of gene fusions in Pseudomonas putida and construction of versatile broad-host-range vectors for direct subcloning of Mu d1 and Mu d2 fusions.

Applied and environmental microbiology ·Vol. 53 ·No. 7 ·1987-07-00 ·Pages 1649-54

Simon V, Schumann W

Abstract

The Mu d1 and Mu d2 prophages were integrated into the conjugative broad-host-range plasmid R751. The two plasmids were then transferred into Pseudomonas putida, and derivatives carrying intact Mu prophages were recovered. After induction of Mu at 42 degrees C, both operon and gene fusions were observed on 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) plates. Broad-host-range vectors were constructed which allow direct cloning of both operon or gene fusions and their analysis in Escherichia coli and P. putida. By using one of these vectors, two operon fusions were isolated from the P. putida chromosome and comparatively analyzed in E. coli and P. putida.

MeSH Terms
Bacteriophages/genetics Cloning, Molecular Conjugation, Genetic DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial/genetics Deoxyribonuclease BamHI Escherichia coli/genetics Genes, Bacterial Genetic Vectors Nucleic Acid Hybridization Operon Plasmids Protein Biosynthesis Pseudomonas/genetics Transcription, Genetic
Chemicals
DNA Transposable Elements DNA, Bacterial DNA Restriction Enzymes Deoxyribonuclease BamHI
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Simon V
Lehrstuhl für Genetik, Universität Bayreuth, Federal Republic of Germany.
Schumann W
References (29)
29 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. Segregation of Lambda Lysogenicity during Bacterial Recombination in Escherichia Coli K12.
    Genetics. 1954 Jul;39(4):429-39 PMID: 17247494
  3. Tn402: a new transposable element determining trimethoprim resistance that inserts in bacteriophage lambda.
    J Bacteriol. 1977 Mar;129(3):1632-5 PMID: 321437
  4. In vivo formation of gene fusions encoding hybrid beta-galactosidase proteins in one step with a transposable Mu-lac transducing phage.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):535-9 PMID: 6320194
  5. Mutagenesis and mutation transfer induced by ultraviolet light in plasmid-cloned DNA.
    Gene. 1984 Feb;27(2):213-22 PMID: 6373503
  6. Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors.
    Gene. 1983 Dec;26(2-3):273-82 PMID: 6323265
  7. [Processes of conjugation and recombination in Escherichia coli. I. Induction by conjugation or zygotic induction].
    Ann Inst Pasteur (Paris). 1956 Oct;91(4):486-510 PMID: 13373067
  8. Analysis of regulation of Klebsiella pneumoniae nitrogen fixation (nif) gene cluster with gene fusions.
    Nature. 1980 Jul 10;286(5769):128-32 PMID: 6995849
  9. Nucleotide sequence of a DNA segment promoting transcription in Pseudomonas putida.
    J Bacteriol. 1986 Jun;166(3):739-45 PMID: 3011741
  10. Isolation of ara-lac gene fusions in Salmonella typhimurium LT2 by using transducing bacteriophage Mu d (Apr lac).
    J Bacteriol. 1980 Sep;143(3):1325-31 PMID: 6773928
  11. Overproduction of the Tn3 transposition protein and its role in DNA transposition.
    Cell. 1982 Feb;28(2):345-54 PMID: 6277510
  12. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  13. Infection of Salmonella typhimurium with coliphage Mu d1 (Apr lac): construction of pyr::lac gene fusions.
    J Bacteriol. 1981 Jan;145(1):299-305 PMID: 6450746
  14. Insertions of transposon Tn5 into ribosomal protein PNA polymerase operons.
    J Bacteriol. 1982 Dec;152(3):1022-32 PMID: 6292159
  15. Introduction of bacteriophage Mu into bacteria of various genera and intergeneric gene transfer by RP4::Mu.
    J Bacteriol. 1981 Jan;145(1):358-68 PMID: 6450749
  16. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  17. A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance.
    Mol Gen Genet. 1979;177(1):65-72 PMID: 231729
  18. Promoter mapping and selection of operator mutants by using insertion of bacteriophage Mu in the argECBH divergent operon of Escherichia coli K-12.
    J Bacteriol. 1982 Jul;151(1):62-7 PMID: 6211434
  19. Trimethoprim R factors in enterobacteria from clinical specimens.
    J Med Microbiol. 1974 May;7(2):169-77 PMID: 4599661
  20. Transposition protein of Tn3: identification and characterisation of an essential repressor-controlled gene product.
    Nature. 1979 Dec 20-27;282(5741):801-6 PMID: 390402
  21. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530-3 PMID: 159458
  22. Expression of the lactose transposon Tn951 in Escherichia coli, Proteus and Pseudomonas.
    J Gen Microbiol. 1980 Jul;119(1):257-62 PMID: 6251161
  23. Specific action of sodium dodecyl sulfate on the sex factor of Escherichia coli K-12 Hfr strains.
    J Bacteriol. 1969 Nov;100(2):827-35 PMID: 4901363
  24. Host: vector systems for gene cloning in Pseudomonas.
    Curr Top Microbiol Immunol. 1982;96:47-67 PMID: 6276095
  25. Uses of lac fusions for the study of biological problems.
    Microbiol Rev. 1985 Dec;49(4):398-418 PMID: 3005818
  26. Plasmid screening at high colony density.
    Gene. 1980 Jun;10(1):63-7 PMID: 6997135
  27. Bacterial bioluminescence: isolation and genetic analysis of functions from Vibrio fischeri.
    Cell. 1983 Mar;32(3):773-81 PMID: 6831560
  28. In vitro constructed plasmids containing both ends of bacteriophage Mu DNA express phage functions.
    Mol Gen Genet. 1979 Jan 16;169(1):97-105 PMID: 286147
  29. A new insertion sequence, IS121, is found on the Mu dI1 (Ap lac) bacteriophage and the Escherichia coli K-12 chromosome.
    J Bacteriol. 1983 Nov;156(2):669-79 PMID: 6313612
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.53.7.1649-1654.1987
Published
1987-07-00
Pages
1649-54
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC203925
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.53.7.1649-1654.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2