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PMID: 2823227 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The inability of the Psammechinus miliaris H3 RNA to be processed in the Xenopus oocyte is associated with sequences distinct from those highly conserved amongst sea urchin histone RNAs.

Nucleic acids research ·Vol. 15 ·No. 20 ·1987-10-26 ·Pages 8305-17

Schaufele F, Birnstiel ML

Abstract

3' processing of precursors of the H3 RNA of the sea urchin Psammechinus miliaris in Xenopus oocytes is dependent upon sea urchin U7 snRNA. Sequences necessary for this interaction are highly conserved in all sea urchin histone precursor RNAs (including the Psammechinus H3) which, in contrast, are efficiently processed in the Xenopus oocyte without the addition of the homologous U7 snRNA. We resolve this seeming paradox by demonstrating here that the inability of the sea urchin Psammechinus miliaris H3 histone RNA to be processed in the Xenopus oocyte is associated with nucleotides immediately 3' to the conserved downstream sea urchin histone sequence element. Thus, a sequence-specific element (or lack of it) is responsible for the poor recognition of the Psammechinus H3 precursor RNA by the Xenopus processing machinery.

MeSH Terms
Animals Cloning, Molecular DNA Restriction Enzymes Female Histones/genetics Oocytes/metabolism Plasmids RNA Precursors/genetics RNA Processing, Post-Transcriptional RNA, Messenger/genetics RNA, Small Nuclear/genetics Sea Urchins/genetics Xenopus
Chemicals
Histones RNA Precursors RNA, Messenger RNA, Small Nuclear DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schaufele F
Institut für Molekularbiologie II, Universität Zürich, Switzerland.
Birnstiel M L
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.20.8305
Published
1987-10-26
Pages
8305-17
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306361
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.20.8305 PMC Full Text View journal details
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