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PMID: 2826333 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Restriction of plasmid DNA during transformation but not conjugation in Neisseria gonorrhoeae.

Infection and immunity ·Vol. 56 ·No. 1 ·1988-01-00 ·Pages 112-6

Stein DC, Gregoire S, Piekarowicz A

Abstract

Neisseria gonorrhoeae strains WR302 and PGH3-2 were characterized with respect to their restriction-modification phenotype. WR302 DNA was cleaved by HaeIII, indicating the lack of methylation at the GGCC sequence. PGH3-2 produced NgoSI (an isoschizomer of NgoII). WR302 produced a restriction enzyme with a recognition sequence different from that of NgoI, NgoII, or NgoIII. Plasmid pFT180 isolated from WR302 was unable to transform PGH3-2, whereas plasmid pFT180 isolated from PGH3-2 was able to transform PGH3-2 at a very high frequency. When plasmid pFT180 isolated from WR302 was methylated in vitro with meth M. HaeIII, this plasmid was able to transform PGH3-2. NgoSI was able to restrict WR302 DNA in vitro, whereas it was incapable of restricting PGH3-2 DNA in vitro. When the self-transmissible R factor pFT6 was mobilized from WR302 to PGH3-2 by conjugation, a 1-order-of-magnitude difference in transfer frequencies was observed, as compared with an isogenic cross. The data indicate that host-mediated restriction can prevent the gonococcus from acquiring DNA via transformation but not via conjugation.

MeSH Terms
Conjugation, Genetic DNA Restriction Enzymes/biosynthesis DNA, Bacterial/metabolism Methylation Neisseria gonorrhoeae/enzymology,genetics Plasmids Transformation, Bacterial
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stein D C
Department of Microbiology, University of Maryland, College Park 20742.
Gregoire S
Piekarowicz A
References (20)
20 references, click to expand
  1. General method for the isolation of plasmid deoxyribonucleic acid.
    J Bacteriol. 1973 Nov;116(2):1064-6 PMID: 4583233
  2. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION.
    J Bacteriol. 1963 Jun;85:1274-9 PMID: 14047217
  3. Identification of a new sequence-specific endonuclease, NgoII, from Neisseria gonorrhoeae.
    J Bacteriol. 1978 Jul;135(1):270-3 PMID: 97270
  4. Transformation-derived Neisseria gonorrhoeae plasmids with altered structure and function.
    J Bacteriol. 1979 May;138(2):510-8 PMID: 108261
  5. Construction and characterization of a new shuttle vector, pLES2, capable of functioning in Escherichia coli and Neisseria gonorrhoeae.
    Gene. 1983 Nov;25(2-3):241-7 PMID: 6319238
  6. Cloning genes for proline biosynthesis from Neisseria gonorrhoeae: identification by interspecific complementation of Escherichia coli mutants.
    J Bacteriol. 1984 May;158(2):696-700 PMID: 6327618
  7. Heterogeneity of molecular size and antigenic expression within lipooligosaccharides of individual strains of Neisseria gonorrhoeae and Neisseria meningitidis.
    Infect Immun. 1984 Sep;45(3):544-9 PMID: 6432693
  8. Entry of double-stranded deoxyribonucleic acid during transformation of Neisseria gonorrhoeae.
    J Bacteriol. 1981 Jan;145(1):638-40 PMID: 6780524
  9. Deoxyribonucleic acid modifications and restriction endonuclease production in Neisseria gonorrhoeae.
    J Bacteriol. 1981 Feb;145(2):788-95 PMID: 6257650
  10. Gonococcal pilus vaccine. Studies of antigenicity and inhibition of attachment.
    J Clin Invest. 1981 Oct;68(4):881-8 PMID: 6116723
  11. Sequence-specific DNA uptake in transformation of Neisseria gonorrhoeae.
    J Bacteriol. 1982 Dec;152(3):1071-7 PMID: 6292160
  12. Characterization of a chimeric beta-lactamase plasmid of Neisseria gonorrhoeae which can function in Escherichia coli.
    Mol Gen Genet. 1983;189(1):77-84 PMID: 6304463
  13. Sequence-specific DNA modification in Neisseria gonorrhoeae.
    J Bacteriol. 1983 Sep;155(3):1324-32 PMID: 6411687
  14. Bacteriophage survival: multiple mechanisms for avoiding the deoxyribonucleic acid restriction systems of their hosts.
    Microbiol Rev. 1983 Sep;47(3):345-60 PMID: 6314109
  15. Genetic transformation of Neisseria gonorrhoeae to streptomycin resistance.
    J Bacteriol. 1966 Nov;92(5):1364-71 PMID: 4958881
  16. Type III 5-methylcytosine modification of DNA in Neisseria gonorrhoeae.
    J Bacteriol. 1985 Mar;161(3):1236-7 PMID: 3918988
  17. Restriction and modification enzymes and their recognition sequences.
    Nucleic Acids Res. 1985;13 Suppl:r165-200 PMID: 2987885
  18. Elaboration of a 3.6-kilodalton lipooligosaccharide, antibody against which is absent from human sera, is associated with serum resistance of Neisseria gonorrhoeae.
    Infect Immun. 1985 Dec;50(3):672-7 PMID: 3934078
  19. Host specificity of DNA produced by Escherichia coli. II. Control over acceptance of DNA from infecting phage lambda.
    J Mol Biol. 1962 Jul;5:37-49 PMID: 13888713
  20. Conjugative plasmids in Neisseria gonorrhoeae.
    J Bacteriol. 1978 Apr;134(1):278-86 PMID: 418060
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.1.112-116.1988
Published
1988-01-00
Pages
112-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259243
Subset
IM
Grants
NCRR NIH HHS · RR-07042 · United States
External Links
PubMed source DOI: 10.1128/iai.56.1.112-116.1988 PMC Full Text View journal details
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