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PMID: 2827496 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Demonstration of glucose-6-phosphatase and peroxisomal catalase activity by ultrastructural cytochemistry in oval cells from livers of carcinogen-treated rats.

The American journal of pathology ·Vol. 130 ·No. 1 ·1988-01-00 ·Pages 91-102

Plenat F, Braun L, Fausto N

Abstract

Oval cells isolated from livers of carcinogen-treated rats have morphologic and biochemical features of immature hepatocytes but seem to lack glucose-6-phosphatase (G6Pase) activity. The authors reinvestigated this question using histochemical methods for visualization of G6Pase activity by light and electron microscopy and the polyene antibiotic filipin to facilitate the penetration of the substrate. Oval cells that proliferate in the liver of animals receiving a carcinogenic diet (choline-deficient, containing 0.05% ethionine) contained G6Pase activity; 50-60% of nonparenchymal epithelial cells isolated from these livers by centrifugal elutriation contained G6Pase activity; and oval cell cultures displayed intense G6Pase activity at confluence but did not have detectable enzyme activity during exponential growth. These results and the demonstration that clofibrate induces peroxisomal proliferation in cultured oval cells strengthen the view that oval cells (or some subpopulation of cells in this compartment) are part of the hepatocyte lineage.

MeSH Terms
Animals Carcinogens Catalase/metabolism Cells, Cultured Clofibrate/toxicity Epithelial Cells Epithelium/enzymology,ultrastructure Freeze Fracturing Glucose-6-Phosphatase/metabolism Liver/drug effects,enzymology,pathology Liver Neoplasms, Experimental/enzymology,pathology,ultrastructure Male Microbodies/enzymology Microscopy, Electron Rats Rats, Inbred Strains
Chemicals
Carcinogens Catalase Glucose-6-Phosphatase Clofibrate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Plenat F
Department of Pathology, Brown University, Providence, RI 02912.
Braun L
Fausto N
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1988-01-00
Pages
91-102
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1880549
Subset
IM
Grants
NCI NIH HHS · CA 23226 · United States
NCI NIH HHS · CA 35249 · United States
NCI NIH HHS · CA07763 · United States
External Links
PubMed source PMC Full Text View journal details
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