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PMID: 2829178 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structural alteration in the MYB protooncogene and deletion within the gene encoding alpha-type protein kinase C in human melanoma cell lines.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 1 ·1988-01-00 ·Pages 74-8

Linnenbach AJ, Huebner K, Reddy EP, Herlyn M, Parmiter AH, Nowell PC, Koprowski H

Abstract

A correlative study was done to determine possible relationships between nonrandom aberrations in chromosomes 1, 6, and 7 occurring in human cutaneous malignant melanoma and the structure of oncogenes as well as specific genes encoding growth factors and growth factor receptors. Thirty cell lines derived from primary or metastatic melanomas of 28 patients were analyzed by Southern blotting with nick-translated probes for 28 different genes, some of which map near frequent chromosomal breakpoints observed in melanoma. An alteration in the MYB protooncogene was observed in a cell line derived from a primary melanoma in the vertical growth phase, which correlated with a 6q22 chromosomal abnormality. Another primary melanoma cell line had a cytogenetically undetected tumor-specific deletion within the gene for alpha-type protein kinase C. Polymorphic alleles for the genes encoding the epidermal growth factor receptor and alpha-type protein kinase C were also observed.

MeSH Terms
Cell Line Chromosome Aberrations Chromosome Deletion Chromosomes, Human, Pair 6 DNA Restriction Enzymes DNA, Neoplasm/genetics Genes Haplotypes Humans Melanoma/enzymology,genetics Nucleic Acid Hybridization Protein Kinase C/genetics Proto-Oncogenes
Chemicals
DNA, Neoplasm Protein Kinase C DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Linnenbach A J
Wistar Institute, Philadelphia, PA 19104.
Huebner K
Reddy E P
Herlyn M
Parmiter A H
Nowell P C
Koprowski H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.1.74
Published
1988-01-00
Pages
74-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279484
Subset
IM
Grants
NCI NIH HHS · CA10815 · United States
NCI NIH HHS · CA21124 · United States
NCI NIH HHS · CA25874 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.1.74 PMC Full Text View journal details
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