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PMID: 2830487 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Alternative splicing of chicken fibronectin in embryos and in normal and transformed cells.

Molecular and cellular biology ·Vol. 7 ·No. 12 ·1987-12-00 ·Pages 4297-307

Norton PA, Hynes RO

Abstract

To study the alternative splicing of fibronectin during embryogenesis and oncogenic transformation, we isolated cDNA clones of chicken fibronectin. The partial amino acid sequence deduced from sequencing of these clones showed that, overall, chicken fibronectin is approximately 80% identical with mammalian fibronectins. However, two of the three known regions of alternative splicing differed from this average. The V region was significantly more divergent, and RNA from embryonic chicken liver showed a pattern of V exon splicing which was distinct from that seen in human or rat fibronectins. In contrast, the EIIIB segment was very highly conserved (96%). As in mammals, this segment and another (EIIIA) were alternatively spliced in a cell-type-specific fashion. EIIIA+ and EIIIB+ species were almost absent in liver but predominated in total embryo RNA at all times from 2.5 to 11 days postfertilization. We also examined the possible contributions of fibronectin splicing and integrin receptor expression to the loss of fibronectin on oncogenic transformation. We detected little change in fibronectin splicing, other than a slight increase in representation of EIIIB+ species in fibroblasts after transformation by Rous sarcoma virus. It was also established that the overall reduction in fibronectin mRNA level observed after transformation was not accompanied by a decrease in integrin mRNA levels, indicating that fibronectin and integrin receptors are not coordinately regulated at the transcriptional level.

MeSH Terms
Amino Acid Sequence Animals Avian Sarcoma Viruses Base Sequence Cell Transformation, Neoplastic Cell Transformation, Viral Chick Embryo DNA/genetics DNA, Recombinant Fibronectins/genetics Humans Integrins Liver/embryology,metabolism Membrane Glycoproteins/genetics Molecular Sequence Data RNA Splicing RNA, Messenger/genetics Rats
Chemicals
DNA, Recombinant Fibronectins Integrins Membrane Glycoproteins RNA, Messenger DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Norton P A
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Hynes R O
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45 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.12.4297-4307.1987
Published
1987-12-00
Pages
4297-307
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368112
Subset
IM
Grants
NCI NIH HHS · F32 CA08027 · United States
NCI NIH HHS · P01 CA26712 · United States
NCI NIH HHS · R01 CA17007 · United States
Databases
GENBANK
M18092, M21554, M21555
External Links
PubMed source DOI: 10.1128/mcb.7.12.4297-4307.1987 PMC Full Text View journal details
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