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PMID: 3119323 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Organization of the fibronectin gene provides evidence for exon shuffling during evolution.

The EMBO journal ·Vol. 6 ·No. 9 ·1987-09-00 ·Pages 2565-72

Patel RS, Odermatt E, Schwarzbauer JE, Hynes RO

Abstract

We report the organization of the two ends of the rat fibronectin gene which encode the type I and II repeating units of the protein. We show that each of these modular structural units is encoded by a separate exon. Homologous type I and II repeats are known to occur in tissue plasminogen activator, factor XII and a bovine seminal plasma protein. Comparison of these sequences and the exon structures of the fibronectin and tissue plasminogen activator genes indicates that exons encoding type I and type II repeats have reassorted during evolution. We also report analyses of the extreme 5' and 3' ends of the fibronectin gene including the promoter region and the exon encoding the prepro sequence of fibronectin and we show that the gene is transcribed from a single initiation site to a single polyadenylation site. These data provide information pertinent to the transcriptional regulation of the gene, the alternative splicing of the primary transcript and the structure of the primary translation product.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution Cloning, Molecular Exons Factor XII/genetics Fibronectins/genetics Genes Molecular Sequence Data Rats Sequence Homology, Nucleic Acid Tissue Plasminogen Activator/genetics
Chemicals
Fibronectins Factor XII Tissue Plasminogen Activator
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Patel R S
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Odermatt E
Schwarzbauer J E
Hynes R O
References (51)
51 references, click to expand
  1. Patterns of amino acids near signal-sequence cleavage sites.
    Eur J Biochem. 1983 Jun 1;133(1):17-21 PMID: 6852022
  2. Transcriptional control of the fibronectin gene in chick embryo fibroblasts transformed by Rous sarcoma virus.
    J Biol Chem. 1983 May 10;258(9):5787-93 PMID: 6189838
  3. Isolation and characterization of cDNA clones for human and bovine fibronectins.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3218-22 PMID: 6304699
  4. Complex regulation of fibronectin synthesis by cells in culture.
    Am J Physiol. 1983 Jul;245(1):C144-50 PMID: 6869518
  5. Primary structure of PDC-109, a major protein constituent of bovine seminal plasma.
    Biochem Biophys Res Commun. 1983 Jun 29;113(3):861-7 PMID: 6870895
  6. Cell surface interactions with extracellular materials.
    Annu Rev Biochem. 1983;52:761-99 PMID: 6351729
  7. Common evolutionary origin of the fibrin-binding structures of fibronectin and tissue-type plasminogen activator.
    FEBS Lett. 1983 Oct 31;163(1):37-41 PMID: 6685059
  8. Sequence-specific binding of glucocorticoid receptor to MTV DNA at sites within and upstream of the transcribed region.
    Cell. 1983 Dec;35(2 Pt 1):381-92 PMID: 6317184
  9. Three different fibronectin mRNAs arise by alternative splicing within the coding region.
    Cell. 1983 Dec;35(2 Pt 1):421-31 PMID: 6317187
  10. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  11. Characterization of DNA sequences through which cadmium and glucocorticoid hormones induce human metallothionein-IIA gene.
    Nature. 1984 Apr 5-11;308(5959):513-9 PMID: 6323998
  12. Complete primary structure of the collagen-binding domain of bovine fibronectin.
    Eur J Biochem. 1984 Apr 16;140(2):235-43 PMID: 6714232
  13. Regulation of fibronectin biosynthesis by glucocorticoids in human fibrosarcoma cells and normal fibroblasts.
    Cell. 1983 May;33(1):287-96 PMID: 6678610
  14. A single rat fibronectin gene generates three different mRNAs by alternative splicing of a complex exon.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5140-4 PMID: 6089177
  15. The structure of the human tissue-type plasminogen activator gene: correlation of intron and exon structures to functional and structural domains.
    Proc Natl Acad Sci U S A. 1984 Sep;81(17):5355-9 PMID: 6089198
  16. Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli.
    Nature. 1984 Sep 20-26;311(5983):232-5 PMID: 6090927
  17. Expression of a human alpha-globin/fibronectin gene hybrid generates two mRNAs by alternative splicing.
    EMBO J. 1984 Nov;3(11):2511-6 PMID: 6096127
  18. The first intron of the human growth hormone gene contains a binding site for glucocorticoid receptor.
    Proc Natl Acad Sci U S A. 1985 Feb;82(3):699-702 PMID: 2983311
  19. Amino acid sequence of the heavy chain of human alpha-factor XIIa (activated Hageman factor).
    J Biol Chem. 1985 May 10;260(9):5328-41 PMID: 3886654
  20. alpha-Interferon-induced transcription of HLA and metallothionein genes containing homologous upstream sequences.
    Nature. 1985 Apr 18-24;314(6012):637-9 PMID: 3990797
  21. Control of types I and II collagen and fibronectin gene expression in chondrocytes delineated by viral transformation.
    Mol Cell Biol. 1985 May;5(5):1002-8 PMID: 2987670
  22. Structure of the human alpha 1-acid glycoprotein gene: sequence homology with other human acute phase protein genes.
    Nucleic Acids Res. 1985 Jun 11;13(11):3941-52 PMID: 2409529
  23. The PDC-109 protein from bovine seminal plasma is similar to the gelatin-binding domain of bovine fibronectin and a kringle domain of human tissue-type plasminogen activator.
    Biochem Biophys Res Commun. 1985 Aug 15;130(3):1010-4 PMID: 4040757
  24. Primary structure of human fibronectin: differential splicing may generate at least 10 polypeptides from a single gene.
    EMBO J. 1985 Jul;4(7):1755-9 PMID: 2992939
  25. Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins.
    Nature. 1985 Aug 29-Sep 4;316(6031):774-8 PMID: 4041012
  26. Repeating modular structure of the fibronectin gene: relationship to protein structure and subunit variation.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6571-5 PMID: 3863113
  27. Modulation of fibronectin gene activity in chick embryo fibroblasts transformed by a temperature-sensitive strain (ts68) of Rous sarcoma virus.
    Nucleic Acids Res. 1985 Nov 25;13(22):8275-84 PMID: 2999717
  28. Evolution of the fibronectin gene. Exon structure of cell attachment domain.
    J Biol Chem. 1986 Feb 15;261(5):2113-6 PMID: 3003095
  29. Identification of a common nucleotide sequence in the 3'-untranslated region of mRNA molecules specifying inflammatory mediators.
    Proc Natl Acad Sci U S A. 1986 Mar;83(6):1670-4 PMID: 2419912
  30. Receptors for glucocorticosteroid and progesterone recognize distinct features of a DNA regulatory element.
    Proc Natl Acad Sci U S A. 1986 May;83(9):2817-21 PMID: 3458241
  31. Fibronectins.
    Sci Am. 1986 Jun;254(6):42-51 PMID: 3010451
  32. A new method for predicting signal sequence cleavage sites.
    Nucleic Acids Res. 1986 Jun 11;14(11):4683-90 PMID: 3714490
  33. Exon structure of the collagen-binding domain of human fibronectin.
    FEBS Lett. 1986 Aug 18;204(2):318-22 PMID: 3732519
  34. Different activities of the adenovirus types 5 and 12 E1A regions in transformation with the EJ Ha-ras oncogene.
    J Virol. 1986 Sep;59(3):684-91 PMID: 3735492
  35. Expression of extracellular matrix genes in relation to myogenesis and neoplastic transformation.
    Exp Cell Res. 1986 Oct;166(2):526-34 PMID: 2427350
  36. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6682-6 PMID: 2875459
  37. Fibronectin is synthesized as an acute phase reactant in rat hepatocytes.
    J Clin Chem Clin Biochem. 1986 Aug;24(8):521-8 PMID: 2428916
  38. Human fibronectin is synthesized as a pre-propolypeptide.
    FEBS Lett. 1986 Oct 20;207(1):145-8 PMID: 3770189
  39. Complete primary structure of bovine plasma fibronectin.
    Eur J Biochem. 1986 Dec 1;161(2):441-53 PMID: 3780752
  40. Efficient and stable expression of recombinant fibronectin polypeptides.
    Proc Natl Acad Sci U S A. 1987 Feb;84(3):754-8 PMID: 3027700
  41. Why genes in pieces?
    Nature. 1978 Feb 9;271(5645):501 PMID: 622185
  42. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  43. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  44. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  45. Regulation of the synthesis of extracellular matrix components in chondroblasts transformed by a temperature-sensitive mutant of Rous sarcoma virus.
    Cell. 1982 Sep;30(2):373-84 PMID: 6291767
  46. Fibronectins: multifunctional modular glycoproteins.
    J Cell Biol. 1982 Nov;95(2 Pt 1):369-77 PMID: 6128348
  47. Purification of twelve cyanogen bromide fragments from bovine plasma fibronectin and the amino acid sequence of eight of them. Overlap evidence aligning two plasmic fragments, internal homology in gelatin-binding region and phosphorylation site near C terminus.
    Eur J Biochem. 1982 Nov 15;128(2-3):605-23 PMID: 6759125
  48. Isolation of genomic DNA clones spanning the entire fibronectin gene.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):46-50 PMID: 6572007
  49. Molecular biology of fibronectin.
    Annu Rev Cell Biol. 1985;1:67-90 PMID: 3916323
  50. Expression of human class II major histocompatibility complex antigens using retrovirus vectors.
    Proc Natl Acad Sci U S A. 1987 Apr;84(8):2150-4 PMID: 3031667
  51. Multiple sites of alternative splicing of the rat fibronectin gene transcript.
    EMBO J. 1987 Sep;6(9):2573-80 PMID: 2445560
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-09-00
Pages
2565-72
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553675
Subset
IM
Grants
NCI NIH HHS · P0I CA26712 · United States
Databases
GENBANK
X05831, X05832, X05833, X05834
External Links
PubMed source PMC Full Text View journal details
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