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PMID: 2830840 Published · ppublish English Journal Article

Effect of DNA gyrase inhibitors pefloxacin, five other quinolones, novobiocin, and clorobiocin on Escherichia coli topoisomerase I.

Antimicrobial agents and chemotherapy ·Vol. 31 ·No. 12 ·1987-12-00 ·Pages 1925-8

Tabary X, Moreau N, Dureuil C, Le Goffic F

Abstract

Two coumarins, inhibitors of the B subunit of DNA gyrase, and six quinolones, inhibitors of the A subunit, were tested against Escherichia coli topoisomerase I-catalyzed DNA relaxation. Coumarins had no effect, whereas quinolones were inhibitors of the enzyme. This inhibition was compared with that of DNA gyrase and calf thymus topoisomerase I. The 50% inhibitory concentrations for E. coli topoisomerase I were about one order of magnitude higher than the corresponding values for E. coli DNA gyrase but were far lower than the known values for calf thymus topoisomerase I. There was a good relationship between inhibition of the two prokaryotic topoisomerases and MICs for E. coli, and the quinolones could be ranked in the same order in the three cases.

MeSH Terms
Electrophoresis, Agar Gel Escherichia coli/enzymology Microbial Sensitivity Tests Norfloxacin/analogs & derivatives,pharmacology Novobiocin/analogs & derivatives,pharmacology Pefloxacin Plasmids Quinolines/pharmacology Topoisomerase I Inhibitors Topoisomerase II Inhibitors
Chemicals
Quinolines Topoisomerase I Inhibitors Topoisomerase II Inhibitors Novobiocin Pefloxacin clorobiocin Norfloxacin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tabary X
Centre d'Etudes et de Recherche en Chimie Organique Appliquée, Centre National de la Recherche Scientifique, Thiais, France.
Moreau N
Dureuil C
Le Goffic F
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.31.12.1925
Published
1987-12-00
Pages
1925-8
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC175829
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.31.12.1925 PMC Full Text View journal details
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