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PMID: 2831392 Published · ppublish English Journal Article

Regulatory elements within the murine leukemia virus enhancer regions mediate glucocorticoid responsiveness.

Journal of virology ·Vol. 62 ·No. 4 ·1988-04-00 ·Pages 1314-22

Celander D, Hsu BL, Haseltine WA

Abstract

Enhancer elements within nonleukemogenic (Akv) and T-cell leukemogenic (SL3-3) murine leukemia viruses demonstrate strong cell type preference in transcriptional activity. These transcription elements are additionally regulated by the synthetic glucocorticoid dexamethasone, and this pattern of regulation varies according to cell type. The sequences required for dexamethasone regulation for both Akv and SL3-3 are shown to include a 17-nucleotide consensus sequence previously termed the glucocorticoid response element (GRE). Although the GREs are identical for both viral enhancers, the sequences surrounding these elements differ, as does the spatial arrangement of the GRE sequences with respect to one another. It is proposed that the spatial arrangement of the GREs, as well as their precise sequence context, determines the difference in the response to dexamethasone of the enhancers in different cell types.

MeSH Terms
Acetyltransferases/genetics Base Sequence Chloramphenicol O-Acetyltransferase DNA, Viral/genetics Dexamethasone/pharmacology Enhancer Elements, Genetic/drug effects Gene Expression Regulation HeLa Cells Humans Leukemia Virus, Murine/drug effects,genetics Plasmids Regulatory Sequences, Nucleic Acid Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Transfection
Chemicals
DNA, Viral Dexamethasone Acetyltransferases Chloramphenicol O-Acetyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Celander D
Dana-Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
Hsu B L
Haseltine W A
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48 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.4.1314-1322.1988
Published
1988-04-00
Pages
1314-22
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253143
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.62.4.1314-1322.1988 PMC Full Text View journal details
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