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PMID: 2833615 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A nonstructural viral protein expressed by a recombinant vaccinia virus protects against lethal cytomegalovirus infection.

Journal of virology ·Vol. 62 ·No. 5 ·1988-05-00 ·Pages 1653-8

Jonjić S, del Val M, Keil GM, Reddehase MJ, Koszinowski UH

Abstract

The nonstructural immediate-early protein pp89 of murine cytomegalovirus (MCMV) is the first viral protein synthesized after infection and has a regulatory function in viral gene expression. Despite its localization in the nucleus of infected cells, pp89 is also the dominant antigen recognized by MCMV-specific cytolytic T lymphocytes. The recombinant vaccinia virus MCMV-ieI-VAC, which expresses pp89, was used to study the capacity of this protein to induce protective immunity in BALB/c mice. Vaccination with MCMV-ieI-VAC induced a long-lasting immunity that protected mice against challenge with a lethal dose of MCMV but did not prevent infection and morbidity. In vivo depletion of CD8+ T lymphocytes before challenge completely abrogated the protective immunity. CD8+ T lymphocytes derived from MCMV-ieI-VAC-primed donors and adoptively transferred into sublethally irradiated and MCMV-infected recipients were found to limit viral replication in host tissues, whereas CD4+ T lymphocytes and pp89-specific antiserum had no protective effect. The data demonstrate for the first time that a single nonstructural viral protein can confer protection against a lethal cytolytic infection and that this immunity is entirely mediated by the CD8+ subpopulation of T lymphocytes.

MeSH Terms
Animals Cytomegalovirus Infections/prevention & control Herpesviridae/genetics,metabolism Mice Mice, Inbred BALB C Recombination, Genetic T-Lymphocytes/immunology Vaccination Vaccinia virus/genetics,metabolism Viral Proteins/immunology,pharmacology
Chemicals
Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jonjić S
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
del Val M
Keil G M
Reddehase M J
Koszinowski U H
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.5.1653-1658.1988
Published
1988-05-00
Pages
1653-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253194
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.62.5.1653-1658.1988 PMC Full Text View journal details
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