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PMID: 2836622 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A transcriptionally defective long terminal repeat within an endogenous copy of mouse mammary tumor virus proviral DNA.

Journal of virology ·Vol. 62 ·No. 7 ·1988-07-00 ·Pages 2394-402

Kuo WL, Vilander LR, Huang M, Peterson DO

Abstract

Mouse mammary tumor virus proviral DNA is endogenous to most inbred strains of mice but in many strains is not transcriptionally active. This inactivity may be due to defects in the proviruses themselves or to position effects mediated by DNA sequences flanking the proviral units. The transcriptional competence of long terminal repeats (LTRs) derived from endogenous proviral DNA at genetic loci Mtv-8, Mtv-9, and Mtv-17 of the C57BL/6 mouse strain was examined with a transient transfection assay in which gene expression was monitored by expression of chloramphenicol acetyltransferase. LTRs from Mtv-8 and Mtv-9 were able to direct glucocorticoid-induced chloramphenicol acetyltransferase expression in this assay, while the LTR from Mtv-17 was only about 5% as effective. Analysis of chimeric LTRs indicated that the glucocorticoid-inducible transcriptional enhancer element within the Mtv-17 LTR is active when linked to a functional promoter from Mtv-8, whereas the promoter from Mtv-17 is defective in directing hormone-induced gene expression, even when linked to the Mtv-8 glucocorticoid-responsive enhancer. The DNA sequence of transcriptional control regions of the LTRs of all three endogenous proviral units was determined; this analysis revealed that the source of the defect in Mtv-17 is a single G-to-A transition at position-75 with respect to the site of transcription initiation that resides within the previously defined binding site for the transcription factor nuclear factor 1. Competition experiments with a gel electrophoresis mobility shift assay indicated that the affinity of nuclear factor 1 for DNA derived from Mtv-17 is significantly less than for comparable sequences derived from Mtv-8.

MeSH Terms
Animals Base Sequence DNA, Viral/genetics Enhancer Elements, Genetic Gene Expression Regulation/drug effects Genes, Viral Glucocorticoids/pharmacology Mammary Tumor Virus, Mouse/genetics,isolation & purification Mice Mice, Inbred C57BL/microbiology Molecular Sequence Data Promoter Regions, Genetic/drug effects Proviruses/analysis Recombinant Fusion Proteins/biosynthesis Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
DNA, Viral Glucocorticoids Recombinant Fusion Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kuo W L
Department of Biochemistry & Biophysics, Texas A&M University, College Station 77843-2128.
Vilander L R
Huang M
Peterson D O
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.7.2394-2402.1988
Published
1988-07-00
Pages
2394-402
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253397
Subset
IM
Grants
NCI NIH HHS · CA32695 · United States
External Links
PubMed source DOI: 10.1128/JVI.62.7.2394-2402.1988 PMC Full Text View journal details
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