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PMID: 2821488 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcriptional repression of a hormone-responsive promoter.

Nucleic acids research ·Vol. 15 ·No. 17 ·1987-09-11 ·Pages 6973-89

Morley KL, Toohey MG, Peterson DO

Abstract

The activity of the mouse mammary tumor virus promoter was assessed in various sequence contexts with a transient transfection assay in which promoter activity was determined by way of expression of a linked gene encoding chloramphenicol acetyltransferase, as well as by direct analysis of RNA transcripts. The results indicate that the proviral long terminal repeat contains a negative transcriptional control element in addition to the glucocorticoid-responsive transcriptional enhancer that has been described previously. The negative element is able to function in both orientations and, at least to some extent, at multiple positions with respect to the regulated transcription unit. The effects on gene expression cannot be explained by alterations in transfection efficiency. The element has been localized to a 91 base pair fragment located immediately 5' of binding sites for the glucocorticoid receptor protein that have been defined in vitro. The role of the negative element may be to repress the inherent activity of the proviral promoter in the absence of glucocorticoids, resulting in an increased ratio of gene expression in the presence and absence of hormone.

MeSH Terms
Acetyltransferases/genetics Animals Chloramphenicol O-Acetyltransferase Chromosome Deletion Dexamethasone/pharmacology Genes Genes, Viral Genetic Linkage Introns L Cells/drug effects,enzymology Mammary Tumor Virus, Mouse/drug effects,genetics Mice Plasmids Promoter Regions, Genetic/drug effects Simian virus 40/genetics Thymidine Kinase/genetics Transcription, Genetic/drug effects
Chemicals
Dexamethasone Acetyltransferases Chloramphenicol O-Acetyltransferase Thymidine Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Morley K L
Department of Biochemistry and Biophysics, Texas A&M University, College Station 77843.
Toohey M G
Peterson D O
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.17.6973
Published
1987-09-11
Pages
6973-89
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306187
Subset
IM
Grants
NCI NIH HHS · CA32695 · United States
External Links
PubMed source DOI: 10.1093/nar/15.17.6973 PMC Full Text View journal details
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