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PMID: 2841576 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The vasopressin mRNA poly(A) tract is unusually long and increases during stimulation of vasopressin gene expression in vivo.

Molecular and cellular biology ·Vol. 8 ·No. 6 ·1988-06-00 ·Pages 2267-74

Carrazana EJ, Pasieka KB, Majzoub JA

Abstract

We developed a method, termed an H-blot, by which the poly(A) tract of any specific mRNA may be detected by RNA filter hybridization after its removal from the body of the mRNA by a RNase H-catalyzed endonucleolytic cleavage in the 3' untranslated region. Using this method, we studied the modulation of the length of the poly(A) tract of rat vasopressin mRNA in vivo during changes in the levels of this mRNA resulting from a physiologic stimulus, osmotic stress. The poly(A) tract of hypothalamic vasopressin mRNA in hydrated rats was, quite remarkably, approximately 250 nucleotides in length, in contrast to that of somatostatin mRNA, which was approximately 30 nucleotides long. Vasopressin mRNA poly(A) tail length increased progressively from approximately 250 to approximately 400 nucleotides with the application of the hyperosmotic stimulus and declined to base line after its removal; somatostatin mRNA poly(A) tail length did not change during osmotic stress. The poly(A) tract length of total hypothalamic mRNA was between 35 and 140 nucleotides and also did not change with osmotic stress. Modulation of poly(A) tract length of specific mRNAs during stimulation of gene expression may provide an additional level of genetic regulation.

MeSH Terms
Animals Drinking Electrophoresis, Polyacrylamide Gel Endoribonucleases/metabolism Hypothalamus/analysis Nucleic Acid Hybridization Poly A/analysis,metabolism RNA, Messenger/analysis,metabolism Rats Ribonuclease H Vasopressins/genetics Water Deprivation
Chemicals
RNA, Messenger Vasopressins Poly A Endoribonucleases Ribonuclease H
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Carrazana E J
Department of Medicine, Howard Hughes Medical Institute, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.
Pasieka K B
Majzoub J A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.6.2267-2274.1988
Published
1988-06-00
Pages
2267-74
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363422
Subset
IM
Grants
NHLBI NIH HHS · 1 P50 HL 36568 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.6.2267-2274.1988 PMC Full Text View journal details
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