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PMID: 2843289 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

ProOmpA is stabilized for membrane translocation by either purified E. coli trigger factor or canine signal recognition particle.

Cell ·Vol. 54 ·No. 7 ·1988-09-23 ·Pages 1003-11

Crooke E, Guthrie B, Lecker S, Lill R, Wickner W

Abstract

We have isolated large amounts of E. coli outer-membrane protein A precursor (proOmpA). Purified proOmpA is active in membrane assembly, and this assembly is saturable with respect to the precursor protein. A proOmpA-Sepharose matrix allows affinity isolation of trigger factor, a soluble, 63,000 dalton monomeric protein that stabilizes proOmpA in assembly competent form. Comparison of trigger factor's amino-terminal sequence with those in a computer data bank and with those encoded by sec genes, as well as groEL and heat shock gene dnaK, suggests that trigger factor is encoded by a previously undescribed gene. Trigger factor and proOmpA form a 1:1 complex that can be isolated by gel filtration. Purified canine signal recognition particle (SRP) can also stabilize proOmpA for membrane insertion. This postribosomal activity of SRP suggests a unifying theme in protein translocation mechanisms.

MeSH Terms
Amino Acid Isomerases/isolation & purification,physiology Animals Bacterial Outer Membrane Proteins/isolation & purification,metabolism Bacterial Proteins/isolation & purification,physiology Biological Transport Carrier Proteins/isolation & purification,physiology Chromatography, Liquid Dogs Escherichia coli/metabolism Escherichia coli Proteins Membranes/metabolism Microsomes/metabolism Pancreas/metabolism Peptidylprolyl Isomerase Protein Binding Protein Precursors/isolation & purification,metabolism Ribonucleoproteins/pharmacology Signal Recognition Particle
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Carrier Proteins Escherichia coli Proteins Protein Precursors Ribonucleoproteins Signal Recognition Particle outer membrane protein A precursor (E coli) Amino Acid Isomerases Peptidylprolyl Isomerase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Crooke E
Molecular Biology Institute, University of California, Los Angeles 90024-1570.
Guthrie B
Lecker S
Lill R
Wickner W
References (36)
36 references, click to expand
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(88)90115-8
Published
1988-09-23
Pages
1003-11
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC7133343
Subset
IM
Grants
NIGMS NIH HHS · GM07104-12 · United States
External Links
PubMed source DOI: 10.1016/0092-8674(88)90115-8 PMC Full Text View journal details
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