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PMID: 2843468 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Association of toxic shock toxin-1 determinant with a heterologous insertion at multiple loci in the Staphylococcus aureus chromosome.

Infection and immunity ·Vol. 56 ·No. 10 ·1988-10-00 ·Pages 2702-8

Chu MC, Kreiswirth BN, Pattee PA, Novick RP, Melish ME, James JF

Abstract

Most Staphylococcus aureus strains associated with toxic shock syndrome and producing toxic shock syndrome toxin 1 (TSST-1) require tryptophan because of a genetic defect in tryptophan biosynthesis. The association between TSST-1 production and tryptophan auxotrophy was not correlated with the phage type, the colonization site, or the disease status of the patient from whom the isolate came. Protoplast fusion and transformation mapping located the genetic determinant of TSST-1 production (tst) very close to the trp operon in such strains and very close to tyrB in a Trp+ TSST-1+ strain. Southern blot hybridization of ClaI-restricted chromosomal DNA with a tst-specific probe revealed a common homologous segment in all of the Trp+ strains with tst linked to tyrB. These results confirmed that the tst determinant in Trp- strains is located at one site, whereas in Trp+ TSST-1+ strains the determinant is located elsewhere on the S. aureus chromosome. It is suggested that the TSST-1 determinant is associated with the insertion of a transposonlike segment into several sites on the S. aureus chromosome.

MeSH Terms
Bacterial Toxins Chromosome Mapping Chromosomes, Bacterial/ultrastructure DNA Transposable Elements DNA, Bacterial/genetics Enterotoxins/genetics,toxicity Genes, Bacterial Genetic Linkage Staphylococcus aureus/genetics,pathogenicity Structure-Activity Relationship Superantigens Tryptophan/genetics
Chemicals
Bacterial Toxins DNA Transposable Elements DNA, Bacterial Enterotoxins Superantigens enterotoxin F, Staphylococcal Tryptophan
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chu M C
Division of Vector-Borne Viral Diseases, Centers for Disease Control, Ft. Collins, Colorado 80522-2087.
Kreiswirth B N
Pattee P A
Novick R P
Melish M E
James J F
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.10.2702-2708.1988
Published
1988-10-00
Pages
2702-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259632
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.56.10.2702-2708.1988 PMC Full Text View journal details
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