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PMID: 2848500 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chromosome-encoded beta-lactamases of Citrobacter diversus. Interaction with beta-iodopenicillanate and labelling of the active site.

The Biochemical journal ·Vol. 254 ·No. 3 ·1988-09-15 ·Pages 891-3

Amicosante G, Oratore A, Joris B, Galleni M, Frère JM, Van Beeumen J

Abstract

Both forms of the chromosome-encoded beta-lactamase of Citrobacter diversus react with beta-iodopenicillanate at a rate characteristic of class A beta-lactamases. The active site of form I was labelled with the same reagent. The sequence of the peptide obtained after trypsin hydrolysis is identical with that of a peptide obtained in a similar manner from the chromosome-encoded beta-lactamase of Klebsiella pneumoniae.

MeSH Terms

Amino Acid Sequence Binding Sites Chromosomes, Bacterial/enzymology Citrobacter/enzymology Isoenzymes/antagonists & inhibitors Penicillanic Acid/pharmacology Peptide Fragments/analysis beta-Lactamase Inhibitors

Chemicals

Isoenzymes Peptide Fragments beta-Lactamase Inhibitors Penicillanic Acid 6-iodopenicillanic acid

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Amicosante G

Università degli Studi dell'Aquila Dipartimento di Scienze e Tecnologie Biomediche e di Biometria, Italy.

Oratore A

Joris B

Galleni M

Frère J M

Van Beeumen J

References (8)

8 references, click to expand

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Interaction of beta-iodopenicillanate with the beta-lactamases of Streptomyces albus G and Actinomadura R39.
Biochem J. 1982 Dec 1;207(3):437-44 PMID: 6299270
6-beta-Iodopenicillanate as a probe for the classification of beta-lactamases.
Biochem J. 1986 Nov 1;239(3):575-80 PMID: 3030266
Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties.
Biochem J. 1988 Sep 15;254(3):885-90 PMID: 3264152
The K1 beta-lactamase of Klebsiella pneumoniae.
Biochem J. 1987 Apr 15;243(2):561-7 PMID: 3307765
The active sites of the beta-lactamases of Streptomyces cacaoi and Streptomyces albus G.
Biochem J. 1987 Jun 1;244(2):427-32 PMID: 2822004
Fractionation and characterization of two beta-lactamases in Citrobacter diversus ULA-27 strain by chromatofocusing.
J Chromatogr. 1987 Aug 21;403:366-72 PMID: 3500180
Automated analysis of enzyme inactivation phenomena. Application to beta-lactamases and DD-peptidases.
Biochem Pharmacol. 1987 Jul 15;36(14):2393-403 PMID: 3038122

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2540891

Published

1988-09-15

Pages

891-3

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1135166

Subset

External Links

PubMed source DOI: 10.1042/bj2540891 PMC Full Text View journal details

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Chromosome-encoded beta-lactamases of Citrobacter diversus. Interaction with beta-iodopenicillanate and labelling of the active site.

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