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PMID: 2849113 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequences of the gypsy transposon of Drosophila necessary for its effects on adjacent genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 24 ·1988-12-00 ·Pages 9650-4

Peifer M, Bender W

Abstract

The Drosophila melanogaster transposon gypsy is the cause of numerous spontaneous mutations, most of which are suppressible by mutations in the suppressor of Hairy wing [su(Hw)] locus. We have examined the phenotype of four revertants of the gypsy element-induced mutation bithoraxoid1 (bxd1) and determined the molecular basis of these reversions. All four revertants have undergone deletions within the gypsy element. The altered gypsy element from one of the partial revertants has been cloned. It has a deletion of only 109 base pairs near the 5' end of the gypsy transcription unit. Similar deletion gypsy elements exist elsewhere in the Drosophila genome. We discuss a mechanism by which the 109-base segment might affect the bxd phenotype.

MeSH Terms
Animals Base Sequence Blotting, Southern Chromosome Deletion DNA Transposable Elements Drosophila melanogaster/genetics Molecular Sequence Data Mutation Phenotype
Chemicals
DNA Transposable Elements
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Peifer M
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115.
Bender W
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.24.9650
Published
1988-12-00
Pages
9650-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282826
Subset
IM
Grants
NIGMS NIH HHS · GM28630 · United States
Databases
GENBANK
M23335, M23336, M23337
External Links
PubMed source DOI: 10.1073/pnas.85.24.9650 PMC Full Text View journal details
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