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PMID: 3096713 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

On the molecular mechanism of gypsy-induced mutations at the yellow locus of Drosophila melanogaster.

The EMBO journal ·Vol. 5 ·No. 10 ·1986-10-00 ·Pages 2657-62

Geyer PK, Spana C, Corces VG

Abstract

We determined the nucleotide sequence of genomic DNA corresponding to the yellow gene. The limits of the transcribed region were deduced from sequence analysis of yellow larval and pupal cDNA clones. The yellow transcription unit is simple, composed of two exons which are processed identically in both developmental stages into a mRNA of 1990 bp. The predicted yellow protein has a mol. wt of 60,752 daltons and appears to be a secreted protein having a structural function and not an enzymatic role in pigmentation. We also characterized the spontaneous mutation y2 and a revertant of this allele to investigate the mutagenic effect of the gypsy element inserted into this locus. Our results show that this transposon is inserted at -700 bp and that the y2+ revertant resulted from excision of the gypsy element leaving behind a complete long terminal repeat (LTR). We conclude, therefore, that the gypsy element is neither inserted into a pupal specific intron or regulatory sequence supporting the hypothesis that mutagenesis is a result of transcriptional interference by the gypsy element on the yellow gene.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Chromosome Mapping DNA/analysis Drosophila Proteins Drosophila melanogaster/anatomy & histology,genetics Insect Hormones/genetics Larva Mutation Pupa Transcription, Genetic
Chemicals
Drosophila Proteins Insect Hormones y protein, Drosophila DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Geyer P K
Spana C
Corces V G
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35 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-10-00
Pages
2657-62
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167166
Subset
IM
Grants
NIGMS NIH HHS · GM32036 · United States
NIGMS NIH HHS · GM35463 · United States
Databases
GENBANK
X04427
External Links
PubMed source PMC Full Text View journal details
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