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PMID: 28640668 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Absence of the Fragile X Mental Retardation Protein results in defects of RNA editing of neuronal mRNAs in mouse.

RNA biology ·Vol. 14 ·No. 11 ·2017-00-02 ·Pages 1580-1591

Filippini A, Bonini D, Lacoux C, Pacini L, Zingariello M, Sancillo L, Bosisio D, Salvi V, Mingardi J, La Via L, Zalfa F, Bagni C, Barbon A

Abstract

The fragile X syndrome (FXS), the most common form of inherited intellectual disability, is due to the absence of FMRP, a protein regulating RNA metabolism. Recently, an unexpected function of FMRP in modulating the activity of Adenosine Deaminase Acting on RNA (ADAR) enzymes has been reported both in Drosophila and Zebrafish. ADARs are RNA-binding proteins that increase transcriptional complexity through a post-transcriptional mechanism called RNA editing. To evaluate the ADAR2-FMRP interaction in mammals we analyzed several RNA editing re-coding sites in the fmr1 knockout (KO) mice. Ex vivo and in vitro analysis revealed that absence of FMRP leads to an increase in the editing levels of brain specific mRNAs, indicating that FMRP might act as an inhibitor of editing activity. Proximity Ligation Assay (PLA) in mouse primary cortical neurons and in non-neuronal cells revealed that ADAR2 and FMRP co-localize in the nucleus. The ADAR2-FMRP co-localization was further observed by double-immunogold Electron Microscopy (EM) in the hippocampus. Moreover, ADAR2-FMRP interaction appeared to be RNA independent. Because changes in the editing pattern are associated with neuropsychiatric and neurodevelopmental disorders, we propose that the increased editing observed in the fmr1-KO mice might contribute to the FXS molecular phenotypes.

Keywords
ADAR2 FMRP Fragile X syndrome RNA editing
MeSH Terms
Adenosine Deaminase/genetics,metabolism Animals Cell Nucleus/metabolism,ultrastructure Cerebral Cortex/metabolism,pathology Disease Models, Animal Fragile X Mental Retardation Protein/genetics,metabolism Fragile X Syndrome/genetics,metabolism,pathology Gene Deletion Hippocampus/metabolism,pathology Humans Male Mice Mice, Knockout Neurons/metabolism,pathology Phenotype Primary Cell Culture Protein Binding RNA Editing RNA, Messenger/genetics,metabolism RNA-Binding Proteins/genetics,metabolism
Chemicals
Fmr1 protein, mouse RNA, Messenger RNA-Binding Proteins Fragile X Mental Retardation Protein ADAR2 protein, mouse Adenosine Deaminase
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Filippini Alice
a Biology and Genetic Division; Department of Molecular and Translational Medicine; University of Brescia ; Brescia , Italy.
Bonini Daniela
a Biology and Genetic Division; Department of Molecular and Translational Medicine; University of Brescia ; Brescia , Italy.
Lacoux Caroline
b Department of Biomedicine and Prevention , University of Rome Tor Vergata , Rome , Italy.
Pacini Laura ORCID
b Department of Biomedicine and Prevention , University of Rome Tor Vergata , Rome , Italy.
Zingariello Maria
c Department of Medicine , Campus Bio-Medico University , via Álvaro del Portillo 21, Rome , Italy.
Sancillo Laura
d Department of Medicine and Aging Sciences, Section of Human Morphology , University G. D'Annunzio of Chieti-Pescara , Chieti , Italy.
Bosisio Daniela
e Immunology Unit; Department of Molecular and Translational Medicine; University of Brescia ; Brescia , Italy.
Salvi Valentina
e Immunology Unit; Department of Molecular and Translational Medicine; University of Brescia ; Brescia , Italy.
Mingardi Jessica
a Biology and Genetic Division; Department of Molecular and Translational Medicine; University of Brescia ; Brescia , Italy.
La Via Luca
a Biology and Genetic Division; Department of Molecular and Translational Medicine; University of Brescia ; Brescia , Italy.
Zalfa Francesca
c Department of Medicine , Campus Bio-Medico University , via Álvaro del Portillo 21, Rome , Italy.
Bagni Claudia
b Department of Biomedicine and Prevention , University of Rome Tor Vergata , Rome , Italy. | f VIB Center for the Biology of Disease and Center for Human Genetics , Leuven , Belgium. | g Department of Fundamental Neuroscience , University of Lausanne , Lausanne , Switzerland.
Barbon Alessandro ORCID
a Biology and Genetic Division; Department of Molecular and Translational Medicine; University of Brescia ; Brescia , Italy.
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Article Info
Journal
RNA biology
Abbr.
RNA Biol
ISSN
1555-8584
Published
2017-00-02
Epub
2017-00-05
Pages
1580-1591
Language
English
Region
United States
NLM ID
101235328
PMCID
PMC5785225
Subset
IM
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