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PMID: 287062 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Inhibition of human neuroblastoma DNA polymerase activities by plant lectins and toxins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 5 ·1979-05-00 ·Pages 2218-21

Bhattacharya P, Simet I, Basu S

Abstract

The effects of concanavalin A and ricin (RCAII, Mr 65,000) on [3H]thymidine incorporation into human neuroblastoma IMR-32 DNA showed reduction of total DNA synthesis to 50% and 70% of control, respectively. Two DNA polymerase (DNA nucleotidyltransferase, EC 2.7.7.7.) activities (alpha and beta) involved in the biosynthesis in vitro of DNA were separated by sucrose density gradient centrifugation from IMR-32 cell homogenate. The DNA polymerase alpha activity was also purified by selective precipitation with polyethylene glycol (Mr 6000) followed by agarose-concanavalin A column chromatography. The activities of both DNA polymerases were examined at various concentrations of mutagenic and nonmutagenic plant agglutinins and the toxin ricin. Concanavalin A and ricin specifically inhibited DNA polymerase alpha activity (activity reduced to 19% and 10%, respectively), whereas DNA polymerase beta activity was inhibited (reduced to 16%) by red kidney bean agglutinin (PHA-P).

MeSH Terms
Cell Line Cell-Free System Concanavalin A/pharmacology DNA, Neoplasm/biosynthesis Lectins/pharmacology Neoplasms, Experimental/enzymology Neuroblastoma/enzymology Nucleic Acid Synthesis Inhibitors Ricin/pharmacology
Chemicals
DNA, Neoplasm Lectins Nucleic Acid Synthesis Inhibitors Concanavalin A Ricin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bhattacharya P
Simet I
Basu S
References (31)
31 references, click to expand
  1. Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes.
    Cancer Res. 1960 May;20:462-6 PMID: 14427849
  2. Induction of spleen cell growth and DNA polymerase activity by Corynebacterium parvum.
    Cancer Res. 1978 Jun;38(6):1617-20 PMID: 647675
  3. DNA polymerase activities in differentiating mouse neuroblastoma N-18 cells.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1289-93 PMID: 274718
  4. DNA polymerase alpha, beta and gamma activities in rabbit spleen cell populations stimulated by various doses of concanavalin A.
    Exp Cell Res. 1978 Apr;113(1):57-62 PMID: 639873
  5. A rapid technique to separate DNA polymerase-alpha and -beta activity from a cytosol extract.
    Anal Biochem. 1978 Aug 1;88(2):399-405 PMID: 697016
  6. Eukaryotic DNA polymerases.
    Annu Rev Biochem. 1977;46:25-47 PMID: 332062
  7. Embryonic chicken brain and mouse neuroblastoma cells N1E-115 and N-18 contain an inhibitor of acid deoxyribonuclease.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):842-5 PMID: 265579
  8. Structure and toxicity of pure ricinus agglutinin.
    Biochim Biophys Acta. 1976 Dec 21;451(2):536-48 PMID: 826270
  9. The effects of phytohemagglutinin on the growth and histochemical properties of mammalian cells in tissue culture.
    Anat Rec. 1968 Dec;162(4):459-66 PMID: 5701623
  10. Cell-substrate adhesivity. A determinant of cell motility.
    Exp Cell Res. 1972 Jan;70(1):33-40 PMID: 4332741
  11. DNA polymerases of eukaryotes.
    FEBS Lett. 1975 Dec 15;60(2):233-43 PMID: 58795
  12. Inhibition of marrow granulocytic colony formation by phytohemagglutinin.
    J Cell Physiol. 1973 Oct;82(2):277-83 PMID: 4356679
  13. Differential toxicity on normal and transformed cells in vitro and inhibition of tumour development in vivo by concanavalin A.
    Nature. 1970 Sep 19;227(5264):1244-6 PMID: 4318127
  14. Characterization of two plant lectins from Ricinus communis and their quantitative interaction with a murine lymphoma.
    Biochemistry. 1974 Jan 1;13(1):196-204 PMID: 4202710
  15. On the toxic proteins abrin and ricin. Studies of their binding to and entry into Ehrlich ascites cells.
    J Biol Chem. 1974 Jun 10;249(11):3557-62 PMID: 4208663
  16. The interactions of lectins with animal cell surfaces.
    Int Rev Cytol. 1974;39:89-190 PMID: 4611947
  17. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas.
    J Natl Cancer Inst. 1973 Sep;51(3):883-90 PMID: 4542714
  18. A unifying hypothesis concerning the nature of malignant growth.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):2840-1 PMID: 4507608
  19. Multiple forms of DNA polymerase in mouse myeloma.
    Proc Natl Acad Sci U S A. 1974 Feb;71(2):578-82 PMID: 4521824
  20. Mechanism of cell entry and toxicity of an affinity- purified lectin from Ricinus communis and its differential effects on normal and virus-transformed fibroblasts.
    Cancer Res. 1975 Jan;35(1):144-55 PMID: 162859
  21. Mechanism of action of the toxic lectins abrin and ricin.
    Nature. 1974 Jun 14;249(458):627-31 PMID: 4857870
  22. Isolation and comparison of galactose-binding lectins from Abrus precatorius and Ricinus communis.
    J Biol Chem. 1974 Feb 10;249(3):803-10 PMID: 4811904
  23. Treatment of abrin and ricin with -mercaptoethanol opposite effects on their toxicity in mice and their ability to inhibit protein synthesis in a cell-free system.
    FEBS Lett. 1972 Nov 15;28(1):48-50 PMID: 4646874
  24. Chemical nature of the receptor site for various phytomitogens.
    Biochemistry. 1972 Oct 10;11(21):4000-5 PMID: 4673056
  25. Inhibition of ascites tumor development by concanavalin A.
    Int J Cancer. 1972 Jan 15;9(1):143-9 PMID: 5015663
  26. Studies on the induction of DNA polymerase during transformation of human lymphocytes.
    Cancer Res. 1972 Jan;32(1):107-13 PMID: 5007680
  27. Lectins.
    Ann N Y Acad Sci. 1970 Feb 13;169(1):168-90 PMID: 5263840
  28. Inhibition by phytohemagglutinin of DNA synthesis in cultured mouse lymphomas.
    J Natl Cancer Inst. 1971 Apr;46(4):763-73 PMID: 5573768
  29. Restoration of normal growth by covering of agglutinin sites on tumour cell surface.
    Nature. 1970 Nov 7;228(5271):512-5 PMID: 5472464
  30. Abrin and ricin: new anti-tumour substances.
    Nature. 1970 Jul 18;227(5255):292-3 PMID: 5428202
  31. Effects of phytohemagglutinin and isoproterenol on DNA synthesis in lymphocytes from normal donors and patients with chronic lymphocytic leukemia.
    Cancer Res. 1970 Mar;30(3):717-23 PMID: 5425294
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.5.2218
Published
1979-05-00
Pages
2218-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383569
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.5.2218 PMC Full Text View journal details
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