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PMID: 274718 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

DNA polymerase activities in differentiating mouse neuroblastoma N-18 cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 3 ·1978-03-00 ·Pages 1289-93

Bhattacharya P, Basu S

Abstract

The activities of two DNA polymerases (DNA nucleotidyltransferases) were characterized in mouse neuroblastoma clone N-18 on the basis of their apparent molecular weights (determined by sucrose density gradient centrifugation: polymerase-alpha, 7.5-8 S; polymerase-beta, 3-4 S) and relative inhibition by sulfhydryl-blocking agents. N-Ethylmaleimide (10 mM) and iodoacetamide (1.5 mM) inhibited DNA polymerase-alpha activity completely, whereas only 35-40% inhibition was observed for DNA polymerase-beta under similar conditions. DNA polymerase-alpha activity was reduced 50-70% in N-18 cells that had been induced to differentiate by 4 micro M bromodeoxyuridine, and the low molecular weight DNA polymerase-beta activity remain unchanged. With activated calf thymus DNA as template, only DNA polymerase-alpha activity was stimulated in the presence of added ribonucleotides and purified Escherichia coli RNA polymerase.

MeSH Terms
Bromodeoxyuridine/pharmacology Cell Differentiation/drug effects Cell Line DNA Polymerase I/antagonists & inhibitors,metabolism DNA Polymerase II/antagonists & inhibitors,metabolism DNA, Neoplasm/biosynthesis DNA-Directed DNA Polymerase/metabolism DNA-Directed RNA Polymerases/metabolism Neoplasms, Experimental Neuroblastoma/enzymology Sulfhydryl Reagents/pharmacology
Chemicals
DNA, Neoplasm Sulfhydryl Reagents DNA-Directed RNA Polymerases DNA Polymerase I DNA Polymerase II DNA-Directed DNA Polymerase Bromodeoxyuridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bhattacharya P
Basu S
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48 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.3.1289
Published
1978-03-00
Pages
1289-93
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411456
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.3.1289 PMC Full Text View journal details
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