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PMID: 2897333 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation and nucleotide sequence of the F17-A gene encoding the structural protein of the F17 fimbriae in bovine enterotoxigenic Escherichia coli.

Infection and immunity ·Vol. 56 ·No. 6 ·1988-06-00 ·Pages 1475-84

Lintermans P, Pohl P, Deboeck F, Bertels A, Schlicker C, Vandekerckhove J, Van Damme J, Van Montagu M, De Greve H

Abstract

The genetic determinant for production of the fimbrial F17 adhesive antigen was isolated from a bovine enterotoxigenic Escherichia coli strain. The F17-A gene, coding for the structural component of the F17 fimbrial adhesin, was cloned and sequenced. An open reading frame of 540 base pairs encoding a polypeptide of 180 amino acids, of which the NH2-terminal 21 residues are characteristic of a signal sequence, has been characterized. The mature protein lacks histidine, methionine, and tryptophan. A possible promoter and ribosome binding site as well as a possible site for termination of transcription are proposed. An important homology of the F17-A protein with fimA and papA fimbrial proteins was found. The N-terminal sequence of the mature F17-A pilin is extremely similar to the N-terminal sequence of the G fimbriae identified on human pyelonephritogenic E. coli strains.

MeSH Terms
Amino Acid Sequence Animals Bacterial Proteins Base Sequence Cattle Chromosome Deletion Cloning, Molecular DNA, Bacterial/isolation & purification Enterotoxemia/genetics,microbiology,pathology Escherichia coli/genetics,pathogenicity,ultrastructure Fimbriae, Bacterial/physiology,ultrastructure Genes Genes, Bacterial Molecular Sequence Data
Chemicals
Bacterial Proteins DNA, Bacterial
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Lintermans P
Laboratory of Bacteriology, National Institute for Veterinary Research, Brussels, Belgium.
Pohl P
Deboeck F
Bertels A
Schlicker C
Vandekerckhove J
Van Damme J
Van Montagu M
De Greve H
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.6.1475-1484.1988
Published
1988-06-00
Pages
1475-84
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259424
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.56.6.1475-1484.1988 PMC Full Text View journal details
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