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PMID: 2897356 Published · ppublish English Comparative Study Journal Article

Cloning and nucleotide sequence of the aroA gene of Bordetella pertussis.

Journal of bacteriology ·Vol. 170 ·No. 6 ·1988-06-00 ·Pages 2467-71

Maskell DJ, Morrissey P, Dougan G

Abstract

The aroA locus of Bordetella pertussis, encoding 5-enolpyruvylshikimate 3-phosphate synthase, has been cloned into Escherichia coli by using a cosmid vector. The gene is expressed in E. coli and complemented an E. coli aroA mutant. The nucleotide sequence of the B. pertussis aroA gene was determined and contains an open reading frame encoding 442 amino acids, with a calculated molecular weight for 5-enolpyruvylshikimate 3-phosphate synthase of 46,688. The amino acid sequence derived from the nucleotide sequence shows homology with the published amino acid sequences of aroA gene products of other microorganisms.

MeSH Terms
3-Phosphoshikimate 1-Carboxyvinyltransferase Alkyl and Aryl Transferases Amino Acid Sequence Aspergillus nidulans/enzymology Base Sequence Bordetella pertussis/genetics Cloning, Molecular Escherichia coli/enzymology,genetics Gene Expression Regulation Molecular Sequence Data Mutation Salmonella typhimurium/enzymology Transferases/genetics
Chemicals
Transferases Alkyl and Aryl Transferases 3-Phosphoshikimate 1-Carboxyvinyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Maskell D J
Department of Molecular Biology, Wellcome Research Laboratories, Beckenham, Kent, United Kingdom.
Morrissey P
Dougan G
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.6.2467-2471.1988
Published
1988-06-00
Pages
2467-71
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211157
Subset
IM
Databases
GENBANK
M20023
External Links
PubMed source DOI: 10.1128/jb.170.6.2467-2471.1988 PMC Full Text View journal details
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