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PMID: 2911585 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Heme oxygenase is the major 32-kDa stress protein induced in human skin fibroblasts by UVA radiation, hydrogen peroxide, and sodium arsenite.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 1 ·1989-01-00 ·Pages 99-103

Keyse SM, Tyrrell RM

Abstract

We have shown that UVA (320-380 nm) radiation, hydrogen peroxide, and sodium arsenite induce a stress protein of approximately 32 kDa in human skin fibroblasts. The synthesis and cloning of cDNA from arsenite-induced mRNA populations have now allowed us to unequivocally identify the 32-kDa protein as heme oxygenase. By mRNA analysis we have shown that the heme oxygenase gene is also induced in cultured human skin fibroblasts by UVA radiation, hydrogen peroxide, cadmium chloride, iodoacetamide, and menadione. The known antioxidant properties of heme catabolites taken together with the observation of a high level of induction of the enzyme in cells from an organ not involved in hemoglobin breakdown strongly supports the proposal that the induction of heme oxygenase may be a general response to oxidant stress and constitutes an important cellular defense mechanism against oxidative damage.

MeSH Terms
Amino Acid Sequence Arsenic/pharmacology Arsenites Base Sequence Cell Line Enzyme Induction Fibroblasts/enzymology Genes Heat-Shock Proteins/biosynthesis,genetics Heme Oxygenase (Decyclizing)/biosynthesis,genetics Heme Oxygenase-1/biosynthesis,genetics Humans Hydrogen Peroxide/pharmacology Kinetics Mixed Function Oxygenases/genetics Molecular Sequence Data Molecular Weight Skin/drug effects,enzymology,radiation effects Sodium Compounds Ultraviolet Rays
Chemicals
Arsenites Heat-Shock Proteins Sodium Compounds sodium arsenite Hydrogen Peroxide Mixed Function Oxygenases HMOX1 protein, human Heme Oxygenase (Decyclizing) Heme Oxygenase-1 Arsenic
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Keyse S M
Swiss Institute for Experimental Cancer Research, Epalinges.
Tyrrell R M
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.1.99
Published
1989-01-00
Pages
99-103
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC286411
Subset
IM
Grants
Cancer Research UK · 12053 · United Kingdom
Databases
GENBANK
M23041, M23042
External Links
PubMed source DOI: 10.1073/pnas.86.1.99 PMC Full Text View journal details
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