Home LiteratureArticle Details
PMID: 2931721 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Effect of DNA polymerase I and DNA helicase II on the turnover rate of UvrABC excision nuclease.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 20 ·1985-10-00 ·Pages 6774-8

Husain I, Van Houten B, Thomas DC, Abdel-Monem M, Sancar A

Abstract

UvrABC excision nuclease (UvrA, UvrB, and UvrC proteins) of Escherichia coli removes nucleotide mono- and diadducts from DNA in the form of oligonucleotides 12 or 13 bases long. We find that the purified enzyme dissociates from DNA very slowly, if at all, in the absence of other proteins implicated in excision repair. Addition of DNA polymerase I and helicase II (UvrD protein) to the reaction mixture stimulates the turnover rate of the excision nuclease to a level comparable to that observed in vivo.

MeSH Terms
Adenosine Triphosphatases/metabolism DNA Helicases DNA Polymerase I/metabolism DNA Repair Endodeoxyribonucleases/metabolism Escherichia coli/enzymology,genetics Escherichia coli Proteins Kinetics Models, Genetic
Chemicals
Escherichia coli Proteins DNA Polymerase I Endodeoxyribonucleases endodeoxyribonuclease uvrABC Adenosine Triphosphatases UvrD protein, E coli DNA Helicases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Husain I
Van Houten B
Thomas D C
Abdel-Monem M
Sancar A
References (27)
27 references, click to expand
  1. Identification of the gene for DNA helicase II of Escherichia coli.
    Eur J Biochem. 1983 Dec 15;137(3):573-80 PMID: 6141047
  2. Enzymatic properties of purified Escherichia coli uvrABC proteins.
    Proc Natl Acad Sci U S A. 1983 Oct;80(20):6157-61 PMID: 6312446
  3. A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region.
    Cell. 1983 May;33(1):249-60 PMID: 6380755
  4. Escherichia coli DNA photolyase stimulates uvrABC excision nuclease in vitro.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7397-401 PMID: 6390436
  5. Repair of nonreplicating UV-irradiated DNA: cooperative dark repair by Escherichia coli uvr and phr functions.
    J Bacteriol. 1985 Feb;161(2):602-8 PMID: 3881404
  6. Stimulation of the UvrABC enzyme-catalyzed repair reactions by the UvrD protein (DNA helicase II).
    Nucleic Acids Res. 1985 Mar 11;13(5):1483-92 PMID: 2987825
  7. Amplification and purification of UvrA, UvrB, and UvrC proteins of Escherichia coli.
    J Biol Chem. 1985 Aug 15;260(17):9875-83 PMID: 2991268
  8. Repair of cis-platinum-DNA adducts by ABC excinuclease in vivo and in vitro.
    J Bacteriol. 1985 Sep;163(3):817-23 PMID: 3897194
  9. THE DISAPPEARANCE OF THYMINE DIMERS FROM DNA: AN ERROR-CORRECTING MECHANISM.
    Proc Natl Acad Sci U S A. 1964 Feb;51:226-31 PMID: 14124320
  10. RELEASE OF ULTRAVIOLET LIGHT-INDUCED THYMINE DIMERS FROM DNA IN E. COLI K-12.
    Proc Natl Acad Sci U S A. 1964 Feb;51:293-300 PMID: 14124327
  11. Excision-repair properties of an Escherichia coli mutant deficient in DNA polymerase.
    Nature. 1970 May 23;226(5247):708-10 PMID: 4910149
  12. DNA binding of the lac repressor.
    J Mol Biol. 1968 Jul 14;34(2):365-8 PMID: 4938552
  13. Use of membrane binding technique to study the kinetics of yeast deoxyribonucleic acid photolyase reactions. Formation of enzyme-substrate complexes in the dark and their photolysis.
    Biochemistry. 1974 May 7;13(10):2149-54 PMID: 4597075
  14. Properties of uvrE mutants of Escherichia coli K12. I. Effects of UV irradiation on DNA metabolism.
    Mutat Res. 1974 Dec;25(3):273-9 PMID: 4612354
  15. DNA unwinding enzyme II of Escherichia coli. 1. Purification and characterization of the ATPase activity.
    Eur J Biochem. 1977 Sep 15;79(1):33-8 PMID: 199440
  16. Repair of UV damage in Escherichia coli under non-growth conditions.
    Photochem Photobiol. 1977 Sep;26(3):247-55 PMID: 335412
  17. Defective excision and postreplication repair of UV-damaged DNA in a recL mutant strain of E. coli K-12.
    Mol Gen Genet. 1977 Oct 24;155(3):267-77 PMID: 340883
  18. Effect of the uvrD mutation on excision repair.
    J Bacteriol. 1980 May;142(2):535-46 PMID: 6991479
  19. The effects of lexA101, recB21, recF143 and uvrD3 mutations on liquid-holding recovery in ultraviolet-irradiated Escherichia coli K12 recA56.
    Mutat Res. 1981 Jan;80(1):15-25 PMID: 7010131
  20. Identification of E. coli uvrC protein.
    Nature. 1981 Jul 9;292(5819):171-3 PMID: 6264323
  21. Identification of the uvrA gene product.
    J Mol Biol. 1981 May 5;148(1):45-62 PMID: 6273577
  22. Identification of the uvrB gene product.
    J Mol Biol. 1981 May 5;148(1):63-76 PMID: 6273578
  23. The uvrD gene of E. coli encodes a DNA-dependent ATPase.
    Nature. 1982 Jul 1;298(5869):98-100 PMID: 6123951
  24. DNA repair in Escherichia coli: identification of the uvrD gene product.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5616-20 PMID: 6291053
  25. An in vitro complementation assay for the Escherichia coli uvrD gene product.
    Nucleic Acids Res. 1983 Apr 11;11(7):2193-204 PMID: 6300798
  26. The E. coli uvrD gene product is DNA helicase II.
    Mol Gen Genet. 1983;190(2):265-70 PMID: 6135974
  27. A multicopy phr-plasmid increases the ultraviolet resistance of a recA strain of Escherichia coli.
    Mutat Res. 1984 Jan;131(1):11-8 PMID: 6229697
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.20.6774
Published
1985-10-00
Pages
6774-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390769
Subset
IM
Grants
NIGMS NIH HHS · GM 32833 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.20.6774 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2