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PMID: 3897194 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Repair of cis-platinum-DNA adducts by ABC excinuclease in vivo and in vitro.

Journal of bacteriology ·Vol. 163 ·No. 3 ·1985-09-00 ·Pages 817-23

Husain I, Chaney SG, Sancar A

Abstract

cis-Platinum compounds, which are used in cancer chemotherapy, are thought to exert their effect by damaging DNA. It is known that this damage is partially repaired in Escherichia coli. Using cis-Pt-treated pBR322 DNA as a probe, we investigated the role of nucleotide excision repair in the removal of Pt-DNA adducts. We found that the nucleotide excision pathway was the major mechanism for repairing Pt adducts in transforming plasmid DNA but that a recA-dependent pathway also contributed to plasmid survival. When cis-Pt-damaged pBR322 was treated with the purified nucleotide excision enzyme ABC excinuclease in vitro, a fraction of the adducts was removed by the enzyme; this removal resulted in a corresponding increase in transformation efficiency.

MeSH Terms
Cisplatin/metabolism DNA Repair DNA, Bacterial/metabolism Endodeoxyribonucleases/metabolism Escherichia coli/drug effects,enzymology,genetics Escherichia coli Proteins Kinetics Organoplatinum Compounds/chemical synthesis,pharmacology Phenotype Plasmids/drug effects
Chemicals
DNA, Bacterial Escherichia coli Proteins Organoplatinum Compounds dichloro-1,2-diaminocyclohexane platinum complex Endodeoxyribonucleases endodeoxyribonuclease uvrABC Cisplatin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Husain I
Chaney S G
Sancar A
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.163.3.817-823.1985
Published
1985-09-00
Pages
817-23
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC219204
Subset
IM
Grants
NCI NIH HHS · CA34082 · United States
NIGMS NIH HHS · GM32833 · United States
External Links
PubMed source DOI: 10.1128/jb.163.3.817-823.1985 PMC Full Text View journal details
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