Home LiteratureArticle Details
PMID: 293711 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Processing in vitro of placental peptide hormones by smooth microsomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 12 ·1979-12-00 ·Pages 6152-6

Bielinska M, Rogers G, Rucinsky T, Boime I

Abstract

Rough and smooth microsomes were prepared from ascites tumor cells, rat liver, and bovine adrenal cortex. Proteolytic removal of the signal peptide in pre-placental lactogen and asparagine-linked glycosylation of the alpha subunit of chorionic gonadotropin by these fractions were examined in mRNA-dependent lysates from ascites cells. Both processing steps were performed by smooth microsomes, which was unexpected because it has been presumed that only rough microsomes contain components for ribosomal binding. Thus smooth microsomes are apparently capable of interacting with polysomes bearing secretory nascent chains, and cleavage and asparagine-linked glycosylation activities are present in both rough and smooth endoplasmic reticulum.

MeSH Terms
Adrenal Cortex/ultrastructure Animals Ascites/pathology Cattle Chorionic Gonadotropin/metabolism Glycoproteins/metabolism Hexosyltransferases/metabolism Membrane Proteins/metabolism Microsomes/metabolism Microsomes, Liver/metabolism Molecular Weight Neoplasms, Experimental/ultrastructure Placental Lactogen/metabolism Protein Precursors/metabolism Rats
Chemicals
Chorionic Gonadotropin Glycoproteins Membrane Proteins Protein Precursors Placental Lactogen Hexosyltransferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bielinska M
Rogers G
Rucinsky T
Boime I
References (21)
21 references, click to expand
  1. Recent developments in the measurement of nucleic acids in biological materials. A supplementary review.
    Analyst. 1966 Feb;91(79):78-88 PMID: 5323520
  2. A low-viscosity epoxy resin embedding medium for electron microscopy.
    J Ultrastruct Res. 1969 Jan;26(1):31-43 PMID: 4887011
  3. Membrane-bound ribosomes of myeloma cells. II. Kinetic studies on the entry of newly made ribosomal subunits into the free and the membrane-bound ribosomal particles.
    J Cell Biol. 1975 Oct;67(1):16-24 PMID: 1176528
  4. Electron microscope studies of ascites tumor cells.
    Ann N Y Acad Sci. 1956 Mar 14;63(5):748-73 PMID: 13314433
  5. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY.
    J Cell Biol. 1965 May;25:407-8 PMID: 14287192
  6. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  7. Membrane assembly in vitro: synthesis, glycosylation, and asymmetric insertion of a transmembrane protein.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3278-82 PMID: 198778
  8. Addition of glucosamine and mannose to nascent immunoglobulin heavy chains.
    Biochemistry. 1977 Oct 4;16(20):4490-7 PMID: 410438
  9. mRNA-dependent synthesis of a glycosylated subunit of human chorionic gonadotropin in cell-free extracts derived from ascites tumor cells.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1768-72 PMID: 273908
  10. Processing of placental peptide hormones synthesized in lysates containing membranes derived from tunicamycin-treated ascites tumor cells.
    J Biol Chem. 1978 Oct 25;253(20):7117-9 PMID: 568137
  11. Characterization of the ribosomal binding site in rat liver rough microsomes: ribophorins I and II, two integral membrane proteins related to ribosome binding.
    J Supramol Struct. 1978;8(3):279-302 PMID: 723266
  12. Proteins of rough microsomal membranes related to ribosome binding. I. Identification of ribophorins I and II, membrane proteins characteristics of rough microsomes.
    J Cell Biol. 1978 May;77(2):464-87 PMID: 649658
  13. Release of poly A(+) messenger RNA from rat liver rough microsomes upon disassembly of bound polysomes.
    J Cell Biol. 1977 Aug;74(2):414-27 PMID: 885910
  14. Studies on the attachment of carbohydrate to ovalbumin nascent chains in hen oviduct.
    J Biol Chem. 1976 Sep 25;251(18):5490-5 PMID: 965372
  15. Effects of phenobarbital on the synthesis and degradation of the protein components of rat liver microsomal membranes.
    J Biol Chem. 1969 Apr 25;244(8):2017-26 PMID: 4181161
  16. Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.
    J Cell Biol. 1975 Dec;67(3):852-62 PMID: 811672
  17. Biogenesis of endoplasmic reticulum membranes. II. Synthesis of constitutive microsomal enzymes in developing rat hepatocyte.
    J Cell Biol. 1966 Jul;30(1):97-117 PMID: 4381698
  18. Structural aspects of the membrane of the endoplasmic reticulum.
    Biochim Biophys Acta. 1975 Dec 29;415(4):411-72 PMID: 173395
  19. Murine intracisternal A type particles fail to separate from the membrane of the endoplasmic reticulum.
    J Virol. 1974 Nov;14(5):1304-6 PMID: 4431082
  20. New colorimetric method for the quantitative estimation of phospholipids without acid digestion.
    J Lipid Res. 1973 Nov;14(6):695-7 PMID: 4742564
  21. mRNA-dependent synthesis of authentic precursor to human placental lactogen: conversion to its mature hormone form in ascites cell-free extracts.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1179-83 PMID: 1063399
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.12.6152
Published
1979-12-00
Pages
6152-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411821
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.12.6152 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2