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PMID: 2960519 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mitochondrial porin of Neurospora crassa: cDNA cloning, in vitro expression and import into mitochondria.

The EMBO journal ·Vol. 6 ·No. 9 ·1987-09-00 ·Pages 2627-33

Kleene R, Pfanner N, Pfaller R, Link TA, Sebald W, Neupert W, Tropschug M

Abstract

cDNA encoding porin of Neurospora crassa, the major protein component of the outer mitochondrial membrane, was isolated and the nucleotide sequence was determined. The deduced protein sequence consists of 283 amino acids (29,979 daltons) and shows sequence homology of around 43% to yeast porin; however, no significant homology to bacterial porins was apparent. According to secondary structure predictions, mitochondrial porin consists mainly of membrane-spanning sided beta-sheets. Porin was efficiently synthesized in vitro from the cDNA; this allowed us to study in detail its import into mitochondria. Thereby, three characteristics of import were defined: (i) import depended on the presence of nucleoside triphosphates; (ii) involvement of a proteinaceous receptor-like component on the surface of the mitochondria was demonstrated; (iii) insertion into the outer membrane was resolved into at least two distinct steps: specific binding to high-affinity sites and subsequent assembly to the mature form.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/metabolism Genetic Vectors Membrane Proteins/genetics Mitochondria/metabolism Molecular Sequence Data Neurospora/genetics Neurospora crassa/genetics,metabolism Porins Protein Biosynthesis Protein Conformation Protein Processing, Post-Translational Transcription, Genetic Voltage-Dependent Anion Channels
Chemicals
Membrane Proteins Porins Voltage-Dependent Anion Channels DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kleene R
Institut für Physiologische Chemie, Universität München, FRG.
Pfanner N
Pfaller R
Link T A
Sebald W
Neupert W
Tropschug M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-09-00
Pages
2627-33
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553683
Subset
IM
Databases
GENBANK
X05824
External Links
PubMed source PMC Full Text View journal details
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