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PMID: 6210913 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

5'-Untranslated sequences of two structural genes in the qa gene cluster of Neurospora crassa.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 6 ·1982-03-00 ·Pages 1955-9

Alton NK, Buxton F, Patel V, Giles NH, Vapnek D

Abstract

The coding regions of two genes (qa-2 and qa-3) in the qa gene cluster of Neurospora crassa have been localized by nucleotide sequence analysis combined with data on previously determined NH2-terminal amino acid sequences for the proteins that these genes encode. The start point of transcription for each of these genes has been determined by nuclease S1 mapping experiments with poly(A)+RNA isolated from quinic acid-induced cultures of N. crassa. The sequences of approximately 200 nucleotides 5' to the start point of transcription have been compared with each other and with those of other eukaryotes. The results show that neither of these regions for the qa-2 nor the qa-3 genes share any significant homology with sequences apparently conserved in higher eukaryotic promoters (-25 and -70 regions). However, the qa-2 and qa-3 sequences do show homology with each other in these regions. Comparison of the 5'-flanking regions of these Neurospora genes with those of several Saccharomyces cerevisiae genes reveals a number of similarities in the region preceding the translation initiation codons.

MeSH Terms
Base Sequence Cloning, Molecular DNA, Fungal/genetics Genes Neurospora/genetics Neurospora crassa/genetics Plasmids Protein Biosynthesis Quinic Acid/metabolism RNA, Messenger/genetics Transcription, Genetic
Chemicals
DNA, Fungal RNA, Messenger Quinic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Alton N K
Buxton F
Patel V
Giles N H
Vapnek D
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.6.1955
Published
1982-03-00
Pages
1955-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346100
Subset
IM
Grants
NIGMS NIH HHS · GM27973 · United States
NIGMS NIH HHS · GM28777 · United States
Databases
GENBANK
J01249, J01250
External Links
PubMed source DOI: 10.1073/pnas.79.6.1955 PMC Full Text View journal details
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