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PMID: 2962194 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Heat-labile regulatory factor is required for 3' processing of histone precursor mRNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 24 ·1987-12-00 ·Pages 8937-40

Gick O, Krämer A, Vasserot A, Birnstiel ML

Abstract

In addition to Sm antigen-type small nuclear ribonucleoprotein particle(s) [snRNP(s)], at least one more factor is involved in the in vitro 3' processing of histone precursor mRNAs (pre-mRNAs) in a HeLa cell nuclear extract. This factor can be completely inactivated by mild heat treatment but is resistant to digestion by micrococcal nuclease and is not immunoprecipitated by antisera of the Sm serotype. Both snRNP (the presumed human homologue of the U7 snRNP of the sea urchin) and the heat-labile factor described above show closely similar properties when fractionated on DEAE, heparin, and Mono Q columns. Fractions, after extensive purification, still contain both heat-labile factor and snRNP activity. When analyzed by gel filtration, the heat-labile component distributes bimodally, the smaller component possessing an apparent molecular weight on the order of 40,000, and the larger, of ca. 300,000.

MeSH Terms
HeLa Cells Histones/genetics Hot Temperature Humans Immunologic Techniques In Vitro Techniques Molecular Weight Nuclear Proteins/physiology RNA Processing, Post-Transcriptional RNA, Messenger/genetics Ribonucleoproteins/physiology Ribonucleoproteins, Small Nuclear
Chemicals
Histones Nuclear Proteins RNA, Messenger Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gick O
Institut für Molekularbiologie II der Universität Zürich, Switzerland.
Krämer A
Vasserot A
Birnstiel M L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.24.8937
Published
1987-12-00
Pages
8937-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299666
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.24.8937 PMC Full Text View journal details
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