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PMID: 2964451 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Secretory vesicles externalize the major plasma membrane ATPase in yeast.

The Journal of cell biology ·Vol. 106 ·No. 3 ·1988-03-00 ·Pages 641-8

Holcomb CL, Hansen WJ, Etcheverry T, Schekman R

Abstract

Yeast cell surface growth is accomplished by constitutive secretion and plasma membrane assembly, culminating in the fusion of vesicles with the bud membrane. Coordination of secretion and membrane assembly has been investigated by examining the biogenesis of plasma membrane ATPase (PM ATPase) in secretion-defective (sec) strains of Saccharomyces cerevisiae. PM ATPase is synthesized as a approximately 106-kD polypeptide that is not detectably modified by asparagine-linked glycosylation or proteolysis during transit to the plasma membrane. Export of the PM ATPase requires the secretory pathway. In sec1, a mutant defective in the last step of secretion, large amounts of Golgi-derived vesicles are accumulated. Biochemical characterization of this organelle has demonstrated that PM ATPase and the secretory enzyme, acid phosphatase, are transported in a single vesicle species.

MeSH Terms
Acid Phosphatase/metabolism Ca(2+) Mg(2+)-ATPase/metabolism Cell Membrane/enzymology Centrifugation, Density Gradient Cytoplasmic Granules/enzymology Electrophoresis, Polyacrylamide Gel Golgi Apparatus/enzymology Immunoassay NADPH-Ferrihemoprotein Reductase/metabolism Saccharomyces cerevisiae/enzymology,ultrastructure Spheroplasts/enzymology
Chemicals
NADPH-Ferrihemoprotein Reductase Acid Phosphatase Ca(2+) Mg(2+)-ATPase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Holcomb C L
Department of Biochemistry, University of California, Berkeley 94720.
Hansen W J
Etcheverry T
Schekman R
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43 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.106.3.641
Published
1988-03-00
Pages
641-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115093
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.106.3.641 PMC Full Text View journal details
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