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PMID: 2966143 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Modulation of Escherichia coli RecBCD activity by the bacteriophage lambda Gam and P22 Abc functions.

Journal of bacteriology ·Vol. 170 ·No. 5 ·1988-05-00 ·Pages 2012-21

Poteete AR, Fenton AC, Murphy KC

Abstract

Plasmids that express the bacteriophage lambda gam gene or the P22 abc2 gene (with and without abc1) at controllable levels were placed in Escherichia coli and tested for effects on the activity of RecBCD. Like Gam, Abc2 inhibited the ATP-dependent exonuclease activity of RecBCD, apparently not by binding to DNA. However, Abc2-mediated inhibition was partial, while Gam-mediated inhibition was complete. Both Abc2 and Gam inhibited host system-mediated homologous recombination in a Chi-containing interval in the chromosome of a hybrid lambda phage; Abc2 inhibited it more strongly than Gam. Gam but not Abc2 spared a phage T4 gene 2 mutant from restriction by RecBCD; Abc2 exhibited weak sparing activity in combination with Abc1 and substantial activity in combination with both Abc1 and P22 homologous recombination function Erf. Either Gam or the combination of the lambda recombination functions Exo and Bet was sufficient to induce a mode of plasmid replication that produced linear multimers. The combination of Abc2, Abc1, and Erf also exhibited this activity. However, Erf was inactive, both by itself and in combination with Abc1; Abc2 had weak activity. These results indicate that Gam and Abc2 modulate the activity of RecBCD in significantly different ways. In comparison with lambda Gam, P22 Abc2 has a weak effect on RecBCD nuclease activity but a strong effect on its recombination-promoting activity.

MeSH Terms
Bacteriophage lambda/genetics DNA Replication DNA, Viral/metabolism Escherichia coli/enzymology,genetics Exodeoxyribonuclease V Exodeoxyribonucleases/antagonists & inhibitors,genetics,metabolism Gene Expression Regulation Genes, Bacterial Genes, Viral Plasmids Recombination, Genetic Salmonella Phages/genetics Viral Proteins/biosynthesis,genetics
Chemicals
DNA, Viral Viral Proteins Exodeoxyribonucleases Exodeoxyribonuclease V
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Poteete A R
Department of Molecular Genetics and Microbiology, University of Massachusetts, Worcester 01605.
Fenton A C
Murphy K C
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36 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.5.2012-2021.1988
Published
1988-05-00
Pages
2012-21
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211079
Subset
IM
Grants
NIAID NIH HHS · AI18234 · United States
External Links
PubMed source DOI: 10.1128/jb.170.5.2012-2021.1988 PMC Full Text View journal details
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