Home LiteratureArticle Details
PMID: 2974063 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The lysine residue in the membrane-spanning domain of the beta chain is necessary for cell surface expression of the T cell antigen receptor.

The Journal of experimental medicine ·Vol. 168 ·No. 6 ·1988-12-01 ·Pages 1971-8

Morley BJ, Chin KN, Newton ME, Weiss A

Abstract

The TCR is a complex receptor composed of seven polypeptide chains consisting of a ligand-binding subunit, Ti, and a putative signal-transducing subunit, CD3. Phylogenetically conserved charged amino acid residues within the membrane-spanning domains present in all seven chains of the TCR have been proposed to be important in the association between Ti and CD3. Using a Ti beta chain-deficient mutant of the cell line Jurkat, site-directed mutagenesis and transfection of Ti beta chain cDNA was performed to assess the importance of the lysine residue at position 290 within the membrane-spanning domain of the Ti beta chain to expression of the TCR complex. These studies demonstrated that the lysine residue, and not simply conservation of either basic charge or secondary structure, is important at this position.

MeSH Terms
Blotting, Northern Cell Line Cell Membrane/metabolism Flow Cytometry Humans Lysine Mutation Receptors, Antigen, T-Cell/metabolism Receptors, Antigen, T-Cell, alpha-beta Transfection
Chemicals
Receptors, Antigen, T-Cell Receptors, Antigen, T-Cell, alpha-beta Lysine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Morley B J
Howard Hughes Medical Institute, Department of Medicine, University of California, San Francisco 94143.
Chin K N
Newton M E
Weiss A
References (22)
22 references, click to expand
  1. Prediction of beta-turns.
    Biophys J. 1979 Jun;26(3):367-83 PMID: 262423
  2. The gamma delta T cell receptor.
    Adv Immunol. 1988;43:133-92 PMID: 3055853
  3. A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains.
    Nature. 1984 Mar 8-14;308(5955):145-9 PMID: 6336315
  4. Genomic organization and sequence of T-cell receptor beta-chain constant- and joining-region genes.
    Nature. 1984 Aug 2-8;310(5976):387-91 PMID: 6336329
  5. Sequence and expression of transcripts of the human T-cell receptor beta-chain genes.
    Nature. 1984 Dec 6-12;312(5994):521-4 PMID: 6334237
  6. Cross-linking of human T cell receptor proteins: association between the T cell idiotype beta subunit and the T3 glycoprotein heavy subunit.
    Cell. 1985 Jan;40(1):183-90 PMID: 3871355
  7. Analysis of cDNA clones specific for human T cells and the alpha and beta chains of the T-cell receptor heterodimer from a human T-cell line.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3430-4 PMID: 3873654
  8. Reconstitution of an active surface T3/T-cell antigen receptor by DNA transfer.
    Nature. 1985 Aug 15-21;316(6029):606-9 PMID: 4033759
  9. Transfer of specificity by murine alpha and beta T-cell receptor genes.
    Nature. 1986 Mar 20-26;320(6059):232-8 PMID: 2421164
  10. Two distinct T-cell receptor alpha-chain transcripts in a rabbit T-cell line: implications for allelic exclusion in T cells.
    Proc Natl Acad Sci U S A. 1986 Apr;83(7):2190-4 PMID: 3485798
  11. Expression of T3 in association with a molecule distinct from the T-cell antigen receptor heterodimer.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6998-7002 PMID: 3092224
  12. Specific antigen-Ia activation of transfected human T cells expressing murine Ti alpha beta-human T3 receptor complexes.
    Nature. 1987 Jan 8-14;325(7000):125-30 PMID: 3027582
  13. Structure, function, and serology of the T-cell antigen receptor complex.
    Annu Rev Immunol. 1987;5:503-40 PMID: 2439105
  14. The T cell receptor.
    Science. 1987 Nov 20;238(4830):1073-9 PMID: 3317824
  15. Cell surface molecules and early events involved in human T lymphocyte activation.
    Adv Immunol. 1987;41:1-38 PMID: 2961214
  16. The gamma T-cell antigen receptor.
    J Clin Immunol. 1987 Nov;7(6):429-40 PMID: 3320076
  17. Molecular cloning of the zeta chain of the T cell antigen receptor.
    Science. 1988 Feb 26;239(4843):1018-21 PMID: 3278377
  18. Failure to synthesize the T cell CD3-zeta chain: structure and function of a partial T cell receptor complex.
    Cell. 1988 Jan 15;52(1):85-95 PMID: 3278811
  19. Chimeric alpha 2-,beta 2-adrenergic receptors: delineation of domains involved in effector coupling and ligand binding specificity.
    Science. 1988 Jun 3;240(4857):1310-6 PMID: 2836950
  20. The T cell receptor/CD3 complex: a dynamic protein ensemble.
    Annu Rev Immunol. 1988;6:629-62 PMID: 3289580
  21. Disulfide linkage of the zeta and eta chains of the T cell receptor. Possible identification of two structural classes of receptors.
    J Biol Chem. 1988 Jul 15;263(20):9874-8 PMID: 3290215
  22. Comparison of T3-associated 49- and 43-kilodalton cell surface molecules on individual human T-cell clones: evidence for peptide variability in T-cell receptor structures.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):4104-8 PMID: 6602985
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.168.6.1971
Published
1988-12-01
Pages
1971-8
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189156
Subset
IM
Grants
NIGMS NIH HHS · GM-39553-01 · United States
External Links
PubMed source DOI: 10.1084/jem.168.6.1971 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2