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PMID: 2981326 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential stability of host mRNAs in Friend erythroleukemia cells infected with herpes simplex virus type 1.

Journal of virology ·Vol. 53 ·No. 1 ·1985-01-00 ·Pages 1-6

Mayman BA, Nishioka Y

Abstract

The consequences of herpes simplex virus type 1 infection on cellular macromolecules were investigated in Friend erythroleukemia cells. The patterns of protein synthesis, examined by polyacrylamide gel electrophoresis, demonstrated that by 4 h postinfection the synthesis of many host proteins, with the exception of histones, was inhibited. Examination of the steady-state level of histone H3 mRNA by molecular hybridization of total RNA to a cloned mouse histone H3 complementary DNA probe demonstrated that the ratio of histone H3 mRNA to total RNA remained unchanged for the first 4 h postinfection. In contrast, the steady-state levels of globin and actin mRNAs decreased progressively at early intervals postinfection. Studies on RNA synthesis in isolated nuclei demonstrated that the transcription of the histone H3 gene was inhibited to approximately the same extent as that of actin gene. We concluded that the stabilization of preexisting histone H3 mRNA was responsible for the persistence of H3 mRNA and histone protein synthesis in herpes simplex virus type 1-infected Friend erythroleukemia cells. The possible mechanisms influencing the differential stability of host mRNAs during the course of productive infection with herpes simplex virus type 1 are discussed.

MeSH Terms
Animals Cell Line Cell Transformation, Viral DNA/metabolism DNA Replication Histones/genetics Kinetics Leukemia, Experimental/genetics Mice Nucleic Acid Hybridization Phosphorus Radioisotopes Protein Biosynthesis RNA, Messenger/genetics Simplexvirus/genetics Transcription, Genetic Uridine Triphosphate/metabolism
Chemicals
Histones Phosphorus Radioisotopes RNA, Messenger DNA Uridine Triphosphate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mayman B A
Nishioka Y
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42 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.53.1.1-6.1985
Published
1985-01-00
Pages
1-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254969
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.53.1.1-6.1985 PMC Full Text View journal details
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