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PMID: 2983109 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Protein stabilization explains the gag requirement for transformation of lymphoid cells by Abelson murine leukemia virus.

Journal of virology ·Vol. 54 ·No. 1 ·1985-04-00 ·Pages 123-32

Prywes R, Hoag J, Rosenberg N, Baltimore D

Abstract

The single protein encoded by Abelson murine leukemia virus is a fusion of sequence from the retroviral gag genes with the v-abl sequence. Deletion of most of the gag region from the transforming protein results in a virus capable of transforming fibroblasts but no longer capable of transforming lymphoid cells. Smaller deletions in gag reveal that p15 gag sequences are responsible for this effect, whereas deletion of p12 sequences had no effect on lymphoid transformation. In transformed fibroblasts, p15-deleted and normal proteins had similar activities and subcellular localization. When the p15-deleted genome was introduced into previously transformed lymphoid lines, its protein product exhibited a marked instability. The tyrosine-specific autophosphorylation activity per cell was less than 1/20th that of the nondeleted protein. Although pulse-Ia-beling showed that the p15-deleted protein was synthesized efficiently, immunoblotting demonstrated that its steady-state level was less than 1/10th that of the nondeleted Abelson protein. The specific instability of the p15-deleted protein in lymphoid cells explains the requirement of these sequences for lymphoid but not fibroblast transformation.

MeSH Terms
Abelson murine leukemia virus/genetics,pathogenicity Animals Antigens, Viral/genetics Cell Transformation, Viral Cells, Cultured Chromosome Deletion Gene Expression Regulation Gene Products, gag Genes, Viral Leukemia Virus, Murine/pathogenicity Lymphocytes/microbiology Mice Structure-Activity Relationship Viral Proteins/genetics
Chemicals
Antigens, Viral Gene Products, gag Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Prywes R
Hoag J
Rosenberg N
Baltimore D
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44 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.54.1.123-132.1985
Published
1985-04-00
Pages
123-32
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254769
Subset
IM
Grants
NCI NIH HHS · CA24220 · United States
NCI NIH HHS · CA26717 · United States
External Links
PubMed source DOI: 10.1128/JVI.54.1.123-132.1985 PMC Full Text View journal details
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