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PMID: 2983348 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of a Q subregion gene in the murine major histocompatibility complex.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 5 ·1985-03-00 ·Pages 1503-7

Flaherty L, DiBiase K, Lynes MA, Seidman JG, Weinberger O, Rinchik EM

Abstract

We have used restriction enzyme digests, Southern blot analysis, and gene transfer experiments to identify a class I gene in the Q subregion of the murine major histocompatibility complex. By comparisons of class I genes from Q congeneic strains, five restriction fragment length polymorphisms were identified. Further studies of mutant (Qa-2-) and wild-type (Qa-2+) BALB/c sublines indicated that at least part of the structural or regulatory gene controlling a Q subregion antigen resides on a 3.7-kilobase Xba I DNA fragment and is absent in all tested Qa-2- strains. The spontaneously occurring Qa-2- BALB/cBy mutant appears to have an extensive deletion in this region. The identity of this gene was confirmed by gene transfer experiments as well as by the use of a single-copy probe.

MeSH Terms
Animals Antigens, Surface/genetics DNA Restriction Enzymes Gene Expression Regulation Histocompatibility Antigens Class I Major Histocompatibility Complex Mice Transfection
Chemicals
Antigens, Surface Histocompatibility Antigens Class I Q surface antigens DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Flaherty L
DiBiase K
Lynes M A
Seidman J G
Weinberger O
Rinchik E M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.5.1503
Published
1985-03-00
Pages
1503-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397291
Subset
IM
Grants
NIAID NIH HHS · AI12603 · United States
NIAID NIH HHS · AI19148 · United States
NIAID NIH HHS · R23AI21022 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.5.1503 PMC Full Text View journal details
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