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PMID: 2985542 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Comparison of 10 IncP plasmids: homology in the regions involved in plasmid replication.

Journal of bacteriology ·Vol. 162 ·No. 2 ·1985-05-00 ·Pages 656-60

Chikami GK, Guiney DG, Schmidhauser TJ, Helinski DR

Abstract

We have examined the DNA homology in the replication regions of 10 IncP plasmids independently isolated from several different countries. Two regions of RK2, the best-studied plasmid of this group, are required for vegetative DNA replication: the origin of replication (oriV) and the trfA region, which codes for a gene product necessary for replication. Six of nine IncP plasmids studied were identical to RK2 in the oriV and trfA regions as shown by Southern hybridization. Three P plasmids, R751, R772, and R906, showed weaker homology with the RK2 trfA, region and hybridized to different-sized HaeII fragments than the other six plasmids. R751, R772, and R906 hybridized to the region of the RK2 replication origin which expresses P incompatibility but differed markedly from RK2 and the other six plasmids in the GC-rich region of the origin required for replication. These data indicate that the P-group plasmids can be divided into two subgroups: IncP alpha, which includes the RK2-like plasmids, and IncP beta which includes the R751-like plasmids.

MeSH Terms
DNA Replication DNA Restriction Enzymes DNA, Bacterial/genetics Genes, Bacterial Plasmids Species Specificity
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chikami G K
Guiney D G
Schmidhauser T J
Helinski D R
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23 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.162.2.656-660.1985
Published
1985-05-00
Pages
656-60
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218900
Subset
IM
Grants
NIAID NIH HHS · AI-07036 · United States
External Links
PubMed source DOI: 10.1128/jb.162.2.656-660.1985 PMC Full Text View journal details
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