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PMID: 6306395 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Heteroduplex analysis of P-plasmid evolution: the role of insertion and deletion of transposable elements.

Molecular & general genetics : MGG ·Vol. 189 ·No. 3 ·1983-00-00 ·Pages 390-9

Villarroel R, Hedges RW, Maenhaut R, Leemans J, Engler G, Van Montagu M, Schell J

Abstract

DNA homology of thirteen R-plasmids of group P was examined by heteroduplex analysis and Southern blotting. Ten of these plasmids showed homology for extensive regions including all genes reported as necessary for replication and conjugational transfer. The differences between these plasmids could be explained by gain or loss of DNA sequences, many of which have been shown to be transposons. Of the other three plasmids, two showed unambiguous homology with the typical P-plasmids but this homology was imperfect, implying that these plasmids are products of lines which have evolved separately for long periods. One plasmid failed to produce heteroduplexes with the reference P plasmid.

MeSH Terms
Anti-Bacterial Agents/pharmacology DNA Transposable Elements DNA, Bacterial/genetics Drug Resistance, Microbial Escherichia coli/drug effects,genetics,ultrastructure Nucleic Acid Hybridization R Factors
Chemicals
Anti-Bacterial Agents DNA Transposable Elements DNA, Bacterial
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Villarroel R
Hedges R W
Maenhaut R
Leemans J
Engler G
Van Montagu M
Schell J
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37 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00325900
Published
1983-00-00
Pages
390-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00325900
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