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PMID: 2991752 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Large-scale production of polyoma middle T antigen by using genetically engineered tumors.

Molecular and cellular biology ·Vol. 5 ·No. 7 ·1985-07-00 ·Pages 1795-9

Kaplan DR, Bockus B, Roberts TM, Bolen J, Israel M, Schaffhausen BS

Abstract

A recombinant plasmid containing a metallothionein promoter-polyoma middle T cDNA fusion was constructed and used to transfect NIH 3T3 cells. Transformed cells expressing middle T were injected into nude mice. Within 3 weeks, each mouse produced tumors containing middle T equivalent to that in 250 to 1,000 100-mm dishes of polyomavirus-infected cells. This middle T, partially purified by immunoaffinity chromatography, retained activity as measured by its ability to be phosphorylated in vitro. The combined approach of fusing strong promoters to genes of interest and utilizing nude mice to grow large quantities of cells expressing the gene provides a quick, inexpensive alternative to other expression systems.

MeSH Terms
Animals Antigens, Viral, Tumor/genetics,isolation & purification Genetic Engineering Mice Mice, Nude Molecular Weight Plasmids Polyomavirus/genetics,immunology Tumor Virus Infections/genetics
Chemicals
Antigens, Viral, Tumor
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kaplan D R
Bockus B
Roberts T M
Bolen J
Israel M
Schaffhausen B S
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32 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.7.1795-1799.1985
Published
1985-07-00
Pages
1795-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367301
Subset
IM
Grants
NCI NIH HHS · R01 CA034722 · United States
PHS HHS · 30002 · United States
PHS HHS · 34722 · United States
NIGMS NIH HHS · GM07196-09 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.7.1795-1799.1985 PMC Full Text View journal details
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