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PMID: 2993653 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Biologically active proviral clone of myeloblastosis-associated virus type 1: implications for the genesis of avian myeloblastosis virus.

Journal of virology ·Vol. 56 ·No. 1 ·1985-10-00 ·Pages 240-4

Perbal B, Lipsick JS, Svoboda J, Silva RF, Baluda MA

Abstract

A biologically active myeloblastosis-associated virus (MAV) provirus was cloned from a bacteriophage recombinant library constructed from leukemic chicken myeloblast DNA. The restriction endonuclease map of this clone was consistent with that of a type 1 MAV (MAV-1). Interference assays of virus recovered from cultured chicken embryo fibroblasts after DNA transfection established that the provirus was infectious and confirmed that it belonged to avian retrovirus subgroup A (type 1). Antipeptide antibodies raised against the env-encoded carboxyl terminus of p48myb, the transforming protein of avian myeloblastosis virus, specifically immunoprecipitated the gp37env from quail cells transfected with MAV-1 proviral DNA but not from cells infected with MAV-2. This suggests that MAV-1 rather than MAV-2 is the progenitor helper virus from which avian myeloblastosis virus arose by the transduction of cellular proto-oncogene sequences.

MeSH Terms
Animals Avian Leukosis Virus/genetics Avian Myeloblastosis Virus/genetics,immunology Cells, Cultured Chickens Cloning, Molecular Cross Reactions DNA Restriction Enzymes DNA, Viral/genetics Helper Viruses/genetics,immunology Transfection Viral Envelope Proteins/immunology Viral Proteins/immunology Virus Replication
Chemicals
DNA, Viral Viral Envelope Proteins Viral Proteins DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Perbal B
Lipsick J S
Svoboda J
Silva R F
Baluda M A
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41 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.56.1.240-244.1985
Published
1985-10-00
Pages
240-4
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252511
Subset
IM
Grants
NCI NIH HHS · CA-10197 · United States
External Links
PubMed source DOI: 10.1128/JVI.56.1.240-244.1985 PMC Full Text View journal details
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