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PMID: 2993804 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Organization of the MAL loci of Saccharomyces. Physical identification and functional characterization of three genes at the MAL6 locus.

Molecular & general genetics : MGG ·Vol. 200 ·No. 1 ·1985-00-00 ·Pages 1-8

Cohen JD, Goldenthal MJ, Chow T, Buchferer B, Marmur J

Abstract

We have physically and functionally identified three genes at the MAL6 locus of Saccharomyces carlsbergensis. Using multicopy yeast plasmid vectors, we have subcloned various segments of the entire MAL6 locus. The functional characterization of the MAL6 subcloned regions was determined by (1) analyzing biochemically the levels of MAL-encoded proteins (maltase [alpha-D-glucosidase, E.C. 3.2.1.20] and maltose transport protein) in cells transformed with various MAL6 subclones, and (2) testing the ability of the subclones to complement the maltose fermentation defects of well characterized Mal- mutants in the highly homologous MAL1 locus. The physical homology between MAL6 and MAL1 is in part demonstrated by the gene disruption of MAL1 using subcloned MAL6 DNA sequences. The results demonstrate that the MAL6 locus is a complex of at least three genes: MAL6R, MAL6T and MAL6S. These genes specify, respectively, a regulatory function, a maltose transport activity (presumably the maltose permease) and the structural gene for maltase. The functional organization of the MAL6 locus is thus identical to that which we had previously determined by mutational analysis for the MAL1 locus.

MeSH Terms
ATP-Binding Cassette Transporters Base Sequence Carrier Proteins/genetics Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics Escherichia coli Proteins Genes Genes, Fungal Genotype Glucosidases/genetics Maltose-Binding Proteins Membrane Proteins/genetics Monosaccharide Transport Proteins Mutation Nucleic Acid Hybridization Saccharomyces/genetics alpha-Glucosidases/genetics
Chemicals
ATP-Binding Cassette Transporters Carrier Proteins Escherichia coli Proteins Maltose-Binding Proteins Membrane Proteins Monosaccharide Transport Proteins maltose transport system, E coli DNA Restriction Enzymes Glucosidases alpha-Glucosidases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cohen J D
Goldenthal M J
Chow T
Buchferer B
Marmur J
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19 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00383304
Published
1985-00-00
Pages
1-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · 5T32GM07128 · United States
NIGMS NIH HHS · GM 28572 · United States
NIGMS NIH HHS · GM07001 · United States
External Links
PubMed source DOI: 10.1007/BF00383304
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