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PMID: 299747 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Immunoinhibition of ristocetin-induced platelet aggregation.

The Journal of clinical investigation ·Vol. 59 ·No. 1 ·1977-01-00 ·Pages 143-8

Nachman RL, Jaffe EA, Weksler BB

Abstract

Human platelets washed and fixed in paraformaldehyde aggregate in the presence of the antibiotic ristocetin and normal plasma. This aggregation response is abolished after digestion of the fixed platelets with chymotrypsin. Antisera to fixed washed platelets were produced in rabbits and absorbed with chymotrypsin-treated, fixed washed platelets. Monovalent Fab fragments obtained from the isolated gamma-globulin fractions of the antisera blocked ristocetin-induced aggregation of fixed washed platelets in buffer and normal platelets in platelet-rich plasma. By double-antibody immunoprecipitation, it was shown that the antibody which blocked the ristocetin reaction interacted with a platelet membrane surface protein of mol wt 155,000. The results suggest that the glycoprotein I complex on the surface of the human platelet mediates ristocetin-induced von Willebrand factor-dependent platelet aggregation.

MeSH Terms
Antibodies Blood Platelets/immunology Cell Membrane/immunology Chymotrypsin/pharmacology Factor VIII/immunology Glycoproteins/immunology Immune Sera Platelet Aggregation/drug effects Ristocetin/antagonists & inhibitors von Willebrand Factor
Chemicals
Antibodies Glycoproteins Immune Sera von Willebrand Factor Ristocetin Factor VIII Chymotrypsin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nachman R L
Jaffe E A
Weksler B B
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30 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI108612
Published
1977-01-00
Pages
143-8
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC333341
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI108612 PMC Full Text View journal details
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