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PMID: 2997708 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Nucleotide sequence of the BsuRI restriction-modification system.

Nucleic acids research ·Vol. 13 ·No. 18 ·1985-09-25 ·Pages 6403-21

Kiss A, Posfai G, Keller CC, Venetianer P, Roberts RJ

Abstract

The genes of the 5'-GGCC specific BsuRI restriction-modification system of Bacillus subtilis have been cloned and expressed in E. coli and their nucleotide sequence has been determined. The restriction and modification genes code for polypeptides with calculated molecular weights of 66,314 and 49,642, respectively. Both enzymes are coded by the same DNA strand. The restriction gene is upstream of the methylase gene and the coding regions are separated by 780 bp. Analysis of the RNA transcripts by S1-nuclease mapping indicates that the restriction and modification genes are transcribed from different promoters. Comparison of the amino acid sequences revealed no homology between the BsuRI restriction and modification enzymes. There are, however, regions of homology between the BsuRI methylase and two other GGCC specific modification enzymes, the BspRI and SPR methylases.

MeSH Terms
Bacillus subtilis/enzymology,genetics Base Sequence Cloning, Molecular Codon DNA Restriction Enzymes DNA-Cytosine Methylases Escherichia coli/genetics Genes Genes, Bacterial Methyltransferases/genetics Molecular Weight Plasmids
Chemicals
Codon DNA modification methylase BsuR DNA-Cytosine Methylases Methyltransferases DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kiss A
Posfai G
Keller C C
Venetianer P
Roberts R J
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40 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.18.6403
Published
1985-09-25
Pages
6403-21
Language
English
Region
England
NLM ID
0411011
PMCID
PMC321967
Subset
IM
Databases
GENBANK
X02988
External Links
PubMed source DOI: 10.1093/nar/13.18.6403 PMC Full Text View journal details
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